Evidence map›Paper›PMID 26798494›Full record

ArticleActa naturae

Valproic Acid Increases the Hepatic Differentiation Potential of Salivary Gland Cells.

O S Petrakova, V V Ashapkin, V Y Shtratnikova, L I Kutueva, E A Vorotelyak, M A Borisov, V V Terskikh, I G Gvazava, A V Vasiliev

Open access · hybridAbstract read
PubMed Full text at PMC
In one paragraph

Article in Acta naturae. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 75% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

O S PetrakovaLomonosov Moscow State University, Faculty of Biology, Leninskie Gory, Moscow State University, 1, bld. 12, 119991, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia ; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 119334 , Moscow, Russia.
V V AshapkinBelozersky Institute, Moscow State University, Leninskie Gory, 1/40, 119991, Moscow, Russia.
V Y ShtratnikovaDepartment of bioengineering and bioinformatics, Lomonosov Moscow State University, Leninskie Gory, 1/73, 119991, Moscow, Russia.
L I KutuevaBelozersky Institute, Moscow State University, Leninskie Gory, 1/40, 119991, Moscow, Russia.
E A VorotelyakLomonosov Moscow State University, Faculty of Biology, Leninskie Gory, Moscow State University, 1, bld. 12, 119991, Moscow, Russia ; Pirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia ; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 119334 , Moscow, Russia.
M A BorisovPirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia.
V V TerskikhKoltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 119334 , Moscow, Russia.
I G GvazavaPirogov Russian National Research Medical University, Ostrovitianov str., 1, 117997, Moscow, Russia ; Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 119334 , Moscow, Russia.
A V VasilievKoltsov Institute of Developmental Biology, Russian Academy of Sciences, Vavilova str., 26, 119334 , Moscow, Russia.
Koltzov Institute of Developmental Biology · RULomonosov Moscow State University · RUMoscow State University · TJPirogov Russian National Research Medical University · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The studies of cell plasticity and differentiation abilities are important problems in modern cellular biology. The use of histone deacetylase inhibitor - valproic acid is a promising approach to increasing the differentiation efficiency of various cell types. In this paper we investigate the ability of mouse submandibular salivary gland cells to differentiate into the hepatic direction and the effect of valproic acid on the efficiency of this differentiation. It was shown that the gene expression levels of hepatocyte markers (Aat, Afp, G6p, Pepck, Tat, Cyp3a13) and liver-enriched transcription factors (Hnf-3α, Hnf-3β, Hnf-4α, Hnf-6) were increased after differentiation in salivary gland cells. Valproic acid increases the specificity of hepatic differentiation, reducing the expression levels of the ductal (Krt19, Hhex1, Cyp7a1) and acinar (Ptf1a) markers. After valproic acid exposure, the efficiency of hepatic differentiation also increases, as evidenced by the increase in the gene expression level of Alb and Tdo, and increase in urea production by differentiated cells. No change was found in DNA methylation of the promoter regions of the genes; however, valproic acid treatment and subsequent hepatic differentiation largely affected the histone H3 methylation of liver-enriched genes. Thus, mouse submandibular salivary gland cells are capable of effective differentiation in the hepatic direction. Valproic acid increases the specificity and efficiency of the hepatic differentiation of these cells.

Indexed as

gene expressionhepatic differentiationsubmandibular salivary gland cellsvalproic acid

Identifiers

PMID26798494
PMCPMC4717252
OpenAlexW2958731029

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.