Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal.

E V Grigor'eva, A I Shevchenko, S P Medvedev, N A Mazurok, A I Zhelezova, S M Zakian

Open access · hybridAbstract read

In one paragraph

Article in Acta naturae. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

0.3field-weighted citation impact, top 36% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

Generation of GABAergic striatal neurons by a novel iPSC differentiation protocol enabling scalability and cryopreservation of progenitor cells.Cytotechnology · 2020
Article
Introducing an expanded CAG tract into the huntingtin gene causes a wide spectrum of ultrastructural defects in cultured human cells.PloS one · 2018
Article
A New MicroRNA Cluster Involved in the Reprogramming to a Pluripotent State.Acta naturae
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

E V Grigor'evaFederal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.

A I ShevchenkoFederal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.

S P MedvedevFederal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia ; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.

N A MazurokFederal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia.

A I ZhelezovaFederal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.

S M ZakianFederal Research Center, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 10, 630090, Novosibirsk, Russia ; Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentiev Ave., 8, 630090, Novosibirsk, Russia ; State Research Institute of Circulation Pathology, Ministry of Healthcare of the, Rechkunovskaya Str., 15, 630055, Novosibirsk, Russia ; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.

Institute of Cytology and Genetics · RUSiberian Branch of the Russian Academy of Sciences · RUMinistry of Healthcare · AZNovosibirsk State University · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Every year, the list of mammalian species for which cultures of pluripotent stem cells (PSCs) are generated increases. PSCs are a unique tool for extending the limits of experimental studies and modeling different biological processes. In this work, induced pluripotent stem cells (iPSCs) from the hybrids of common voles Microtus levis and Microtus arvalis, which are used as model objects to study genome organization on the molecular-genetic level and the mechanisms of X-chromosome inactivation, have been generated. Vole iPSCs were isolated and cultured in a medium containing cytokine LIF, basic fibroblast growth factor (bFGF), ascorbic acid, and fetal bovine serum. Undifferentiated state of vole iPSCs is maintained by activation of their endogenous pluripotency genes - Nanog, Oct4, Sox2, Sall4, and Esrrb. The cells were able to maintain undifferentiated state for at least 28 passages without change in their morphology and give rise to three germ layers (ectoderm, mesoderm and endoderm) upon differentiation.

Indexed as

common volesinduced pluripotent stem cellsreprogramming

Identifiers

PMID26798492

PMCPMC4717250

OpenAlexW2410640379

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.