ArticleStem cells international2016
Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation.
Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 77 citations in OpenAlex.
- Changes in neural progenitor lineage composition during astrocytic differentiation of human iPSCs.eLife · 2025Article
- Geometrical constraints dictate assembly and phenotype of human iPSC-derived motoneuronal spheroids.Stem cell research & therapy · 2025Article
- Altered metabolic function induced by Aβ-oligomers and PSEN1 mutations in iPSC-derived astrocytes.Journal of neurochemistry · 2025Article
- Assessment and Evaluation of Contemporary Approaches for Astrocyte Differentiation from hiPSCs: A Modeling Paradigm for Alzheimer's Disease.Biological procedures online · 2024Article
- ASCL1-mediated direct reprogramming: converting ventral midbrain astrocytes into dopaminergic neurons for Parkinson's disease therapy.BMB reports · 2024Article
- Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs).PloS one · 2024Article
- Human iPSC-derived glia models for the study of neuroinflammation.Journal of neuroinflammation · 2023Review
- Human retinal ganglion cell neurons generated by synchronous BMP inhibition and transcription factor mediated reprogramming.NPJ Regenerative medicine · 2023Article
- Article
- Neural fate commitment of rat full-term amniotic fluid stem cellsIBRO neuroscience reports · 2023Article
- Efficient Generation of Dopaminergic Neurons from Mouse Ventral Midbrain Astrocytes.Biomolecules & therapeutics · 2023Article
- iPSC-derived three-dimensional brain organoid models and neurotropic viral infections.Journal of neurovirology · 2023Review
- Insights into Human-Induced Pluripotent Stem Cell-Derived Astrocytes in Neurodegenerative Disorders.Biomolecules · 2022Review
- Review
- Retroviral infection of human neurospheres and use of stem Cell EVs to repair cellular damage.Scientific reports · 2022Article
- Differentiation of human induced pluripotent stem cells into cortical neural stem cells.Frontiers in cell and developmental biology · 2022Article
- Dissecting the complexities of Alzheimer disease with in vitro models of the human brain.Nature reviews. Neurology · 2022Review
- Scientific Validation of Human Neurosphere Assays for Developmental Neurotoxicity Evaluation.Frontiers in toxicology · 2022Article
- Mood Stabilizers in Psychiatric Disorders and Mechanisms Learnt from In Vitro Model Systems.International journal of molecular sciences · 2021Review
- Cellular Models in Schizophrenia Research.International journal of molecular sciences · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) are traditionally maintained and proliferated utilizing two-dimensional (2D) adherent monolayer culture systems. However, NPCs cultured using this system hardly reflect the intrinsic spatial development of brain tissue. In this study, we determined that culturing iPSC-derived NPCs as three-dimensional (3D) floating neurospheres resulted in increased expression of the neural progenitor cell (NPC) markers, PAX6 and NESTIN. Expansion of NPCs in 3D culture methods also resulted in a more homogenous PAX6 expression when compared to 2D culture methods. Furthermore, the 3D propagation method for NPCs resulted in a significant higher expression of the astrocyte markers GFAP and aquaporin 4 (AQP4) in the differentiated cells. Thus, our 3D propagation method could constitute a useful tool to promote NPC homogeneity and also to increase the differentiation potential of iPSC towards astrocytes.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.