Evidence map›Paper›PMID 26798207›Full record

ArticleJournal of proteomics & bioinformatics2015

Pilot Application of Magnetic Nanoparticle-Based Biosensor for Necrotizing Enterocolitis.

Dokyoon Kim, Changlin Fu, Xuefeng B Ling, Zhongkai Hu, Guozhong Tao, Yingzhen Zhao, Zachary J Kastenberg, Karl G Sylvester, Shan X Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of proteomics & bioinformatics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Precision Medicine for Neonatal Sepsis.Frontiers in molecular biosciences · 2018
    Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Dokyoon Kim *Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA.
Changlin Fu *Department of Surgery, Stanford University, Stanford, CA 94305, USA.
Xuefeng B Ling *Department of Surgery, Stanford University, Stanford, CA 94305, USA.
Zhongkai HuDepartment of Surgery, Stanford University, Stanford, CA 94305, USA.
Guozhong TaoDepartment of Surgery, Stanford University, Stanford, CA 94305, USA.
Yingzhen ZhaoDepartment of Surgery, Stanford University, Stanford, CA 94305, USA.
Zachary J KastenbergDepartment of Surgery, Stanford University, Stanford, CA 94305, USA.
Karl G SylvesterDepartment of Surgery, Stanford University, Stanford, CA 94305, USA.
Shan X WangDepartment of Materials Science and Engineering, Stanford University, Stanford, CA 94305, USA.

Funding

TRANS-NETWORK PROJECTSU54CA143907 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI NOLAN, GARRY P · 2009 to 2013
$15.2M
Pilot ProjectsU54CA151459 · NCI · STANFORD UNIVERSITY · PI GAMBHIR, SANJIV S · 2010 to 2014
$12.6M
NCI NIH HHS U54 CA143907NCI NIH HHS U54 CA151459
6 · The paper itself

Abstract

backgroundNecrotizing Enterocolitis (NEC) is a major source of neonatal morbidity and mortality. There is an ongoing need for a sensitive diagnostic instrument to discriminate NEC from neonatal sepsis. We hypothesized that magnetic nanopartile-based biosensor analysis of gut injury-associated biomarkers would provide such an instrument. STUDY

designWe designed a magnetic multiplexed biosensor platform, allowing the parallel plasma analysis of C-reactive protein (CRP), matrix metalloproteinase-7 (MMp7), and epithelial cell adhesion molecule (EpCAM). Neonatal subjects with sepsis (n=5) or NEC (n=10) were compared to control (n=5) subjects to perform a proof of concept pilot study for the diagnosis of NEC using our ultra-sensitive biosensor platform.

resultsOur multiplexed NEC magnetic nanoparticle-based biosensor platform was robust, ultrasensitive (Limit of detection LOD: CRP 0.6 pg/ml; MMp7 20 pg/ml; and EpCAM 20 pg/ml), and displayed no cross-reactivity among analyte reporting regents. To gauge the diagnostic performance, bootstrapping procedure (500 runs) was applied: MMp7 and EpCAM collectively differentiated infants with NEC from control infants with ROC AUC of 0.96, and infants with NEC from those with sepsis with ROC AUC of 1.00. The 3-marker panel comprising of EpCAM, MMp7 and CRP had a corresponding ROC AUC of 0.956 and 0.975, respectively.

conclusionThe exploration of the multiplexed nano-biosensor platform shows promise to deliver an ultrasensitive instrument for the diagnosis of NEC in the clinical setting.

Indexed as

BiosensorDiagnosisNanoparticleNECNecrotizing enterocolitisSepsis

Identifiers

PMID26798207
PMCPMC4718576

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.