ArticleMolecular biotechnology2016
Identification of Suitable Endogenous Normalizers for qRT-PCR Analysis of Plasma microRNA Expression in Essential Hypertension.
Article in Molecular biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Circulating MicroRNAs as Promising Diagnostic Biomarkers for Patients With Glioma: A Meta-Analysis.Frontiers in neurology · 2020Pooled it
- The potential role of serum miR-182-5p and miR-127-5p in diagnosis of metabolic dysfunction-associated steatotic liver disease and its relationship with insulin resistance.Przeglad gastroenterologiczny · 2026Article
- MicroRNA-133a and MicroRNA-145 May Be Involved in the Development of Hypertension-Mediated Organ Damage in Children with Primary Hypertension-A Preliminary Study.Journal of clinical medicine · 2024Article
- Data normalization of plasma miRNA profiling from patients with COVID-19.Scientific reports · 2024Article
- Article
- Potential of miRNAs in Plasma Extracellular Vesicle for the Stratification of Prostate Cancer in a South African Population.Cancers · 2023Article
- Placental expression of RNU44, RNU48 and miR-16-5p: stability and relations with fetoplacental growth.European journal of clinical nutrition · 2022Article
- Placental expression of miR-21-5p, miR-210-3p and miR-141-3p: relation to human fetoplacental growth.European journal of clinical nutrition · 2022Article
- Relationships between plasma expression levels of microRNA-146a and microRNA-132 in epileptic patients and their cognitive, mental and psychological disorders.Bioengineered · 2022Article
- Comparison of Data Normalization Strategies for Array-Based MicroRNA Profiling Experiments and Identification and Validation of Circulating MicroRNAs as Endogenous Controls in Hypertension.Frontiers in genetics · 2022Article
- Article
- Description of a CSF-Enriched miRNA Panel for the Study of Neurological Diseases.Life (Basel, Switzerland) · 2021Article
- Article
- Identification of miR-29c-3p as a Robust Normalizer for Urine microRNA Studies in Bladder Cancer.Biomedicines · 2020Article
- Exosomal microRNAs as a promising theragnostic tool for essential hypertension.Hypertension research : official journal of the Japanese Society of Hypertension · 2020Article
- Identification of miR-328-3p as an endogenous reference gene for the normalization of miRNA expression data from patients with Diabetic Retinopathy.Scientific reports · 2019Article
- MicroRNA expression studies: challenge of selecting reliable reference controls for data normalization.Cellular and molecular life sciences : CMLS · 2019Review
- Identification and validation of circulating miRNAs as endogenous controls in obstructive sleep apnea.PloS one · 2019Article
- microRNA-21 and hypertension.Hypertension research : official journal of the Japanese Society of Hypertension · 2018Review
- Relationship of Circulating miRNAs with Insulin Sensitivity and Associated Metabolic Risk Factors in Humans.Metabolic syndrome and related disorders · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Circulating microRNAs (miRNAs) are promising biomarkers for many diseases. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a gold standard for miRNA expression profiling that requires proper data normalization. Since there is no universal normalizer, it is recommended to evaluate normalizers under every experimental condition. This study describes the identification of suitable endogenous normalizer(s) (ENs) for plasma miRNA expression in essential hypertension. Expression levels of 5 candidate ENs and 2 plasma quality markers were determined by qRT-PCR in plasma samples from 18 hypertensive patients and 10 healthy controls. NormFinder, GeNorm, and DataAssist software programs were used to select the best EN(s). Expression levels of the 5 candidate ENs were also analyzed in urine samples from hypertensive patients and compared to the plasma samples of the hypertensive patients. Among the analyzed candidates, hsa-miR-92a-3p was identified as the best EN, and hsa-miR-21-5p and hsa-miR-16-5p as the next best. Moreover, hsa-miR-92a-3p showed the most consistent expression between plasma and urine. In conclusion, this study showed that hsa-miR-92a-3p, hsa-miR-21-5p, and hsa-miR-16-5p may be used as normalizers for plasma miRNA expression data in essential hypertension studies.
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Registered trials
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