Evidence map›Paper›PMID 26798059›Full record

ArticleStem cells translational medicine2016

Priming Dental Pulp Stem Cells With Fibroblast Growth Factor-2 Increases Angiogenesis of Implanted Tissue-Engineered Constructs Through Hepatocyte Growth Factor and Vascular Endothelial Growth Factor Secretion.

Caroline Gorin, Gael Y Rochefort, Rumeyza Bascetin, Hanru Ying, Julie Lesieur, Jérémy Sadoine, Nathan Beckouche, Sarah Berndt, Anita Novais, Matthieu Lesage and 13 more

Open access · goldAbstract read
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In one paragraph

Article in Stem cells translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
73citing papers in PubMed, 1 pooled it
8.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

73 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.

  1. Pooled it
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  8. NINJ1 impairs the anti-inflammatory function of hUC-MSCs with synergistic IFN-γ and TNF-α stimulation.Chinese journal of traumatology = Zhonghua chuang shang za zhi · 2025
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  15. Matrix stiffness-dependent regulation of immunomodulatory genes in human MSCs is associated with the lncRNA CYTOR.Proceedings of the National Academy of Sciences of the United States of America · 2024
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13 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors at 10 institutions in 1 country.

Caroline GorinEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France Assistance Publique des Hôpitaux de Paris (AP-HP) Département d'Odontologie, Hôpitaux Universitaires PNVS, Paris, France.
Gael Y RochefortEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France.
Rumeyza BascetinCenter for Interdisciplinary Research in Biology, Collège de France, Paris, France Inserm U1050, Paris, France CNRS UMRS 7241, Paris, France.
Hanru YingCenter for Interdisciplinary Research in Biology, Collège de France, Paris, France Inserm U1050, Paris, France CNRS UMRS 7241, Paris, France.
Julie LesieurEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France.
Jérémy SadoineEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France.
Nathan BeckoucheCenter for Interdisciplinary Research in Biology, Collège de France, Paris, France Inserm U1050, Paris, France CNRS UMRS 7241, Paris, France.
Sarah BerndtCenter for Interdisciplinary Research in Biology, Collège de France, Paris, France Inserm U1050, Paris, France CNRS UMRS 7241, Paris, France.
Anita NovaisEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France.
Matthieu LesageCenter for Interdisciplinary Research in Biology, Collège de France, Paris, France Inserm U1050, Paris, France CNRS UMRS 7241, Paris, France.
Benoit HostenINSERM UMR-S1144, Université Paris Descartes-Paris Diderot Sorbonne Paris Cité, AP-HP, Hôpital St. Louis, Unité Claude Kellershohn, Paris, France.
Laetitia VercellinoUniversité Paris Diderot, AP-HP, Hôpital St. Louis, Unité Claude Kellershohn, Paris, France.
Pascal MerletUniversité Paris Diderot, AP-HP, Hôpital St. Louis, Unité Claude Kellershohn, Paris, France.
Dominique Le-DenmatEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France.
Carmen MarchiolInstitut Cochin, Plateforme Imagerie du vivant, Université Paris Descartes Sorbonne Paris Cité, Paris, France.
Didier LetourneurINSERM U1148, Laboratory of Vascular Translational Science, Université Paris Diderot Sorbonne Paris Cité, Sorbonne Paris Cité, Faculté de Médecine, Site Xavier Bichat, and Département Hospitalo-Universitaire Fibrosis, Inflammation, and Remodeling, Paris, France.
Antonino NicolettiINSERM U1148, Laboratory of Vascular Translational Science, Université Paris Diderot Sorbonne Paris Cité, Sorbonne Paris Cité, Faculté de Médecine, Site Xavier Bichat, and Département Hospitalo-Universitaire Fibrosis, Inflammation, and Remodeling, Paris, France.
Sibylle Opsahl VitalEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France Assistance Publique des Hôpitaux de Paris (AP-HP) Département d'Odontologie, Hôpitaux Universitaires PNVS, Paris, France.
Anne PoliardEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France.
Benjamin SalmonEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France Assistance Publique des Hôpitaux de Paris (AP-HP) Département d'Odontologie, Hôpitaux Universitaires PNVS, Paris, France.
Laurent MullerCenter for Interdisciplinary Research in Biology, Collège de France, Paris, France Inserm U1050, Paris, France CNRS UMRS 7241, Paris, France catherine.chaussain@parisdescartes.fr laurent.muller@college-de-france.fr.
Catherine ChaussainEA 2496 Pathologies, Imagerie et Biothérapies orofaciales et Plateforme Imagerie du Vivant, Dental School, Université Paris Descartes Sorbonne Paris Cité, Montrouge, France Assistance Publique des Hôpitaux de Paris (AP-HP) Département d'Odontologie, Hôpitaux Universitaires PNVS, Paris, France catherine.chaussain@parisdescartes.fr laurent.muller@college-de-france.fr.
Stéphane GermainCenter for Interdisciplinary Research in Biology, Collège de France, Paris, France Inserm U1050, Paris, France CNRS UMRS 7241, Paris, France.
Université Paris Cité · FRSorbonne Paris Cité · FRCentre Interdisciplinaire de Recherche en Biologie · FRCentre National de la Recherche Scientifique · FRCollège de France · FRDélégation Paris 7 · FRInserm · FRAssistance Publique – Hôpitaux de Paris · FRHôpitaux Universitaires Paris-Ouest · FRLaboratory Orofacial Pathologies, Imaging and Biotherapies · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tissue engineering strategies based on implanting cellularized biomaterials are promising therapeutic approaches for the reconstruction of large tissue defects. A major hurdle for the reliable establishment of such therapeutic approaches is the lack of rapid blood perfusion of the tissue construct to provide oxygen and nutrients. Numerous sources of mesenchymal stem cells (MSCs) displaying angiogenic potential have been characterized in the past years, including the adult dental pulp. Establishment of efficient strategies for improving angiogenesis in tissue constructs is nevertheless still an important challenge. Hypoxia was proposed as a priming treatment owing to its capacity to enhance the angiogenic potential of stem cells through vascular endothelial growth factor (VEGF) release. The present study aimed to characterize additional key factors regulating the angiogenic capacity of such MSCs, namely, dental pulp stem cells derived from deciduous teeth (SHED). We identified fibroblast growth factor-2 (FGF-2) as a potent inducer of the release of VEGF and hepatocyte growth factor (HGF) by SHED. We found that FGF-2 limited hypoxia-induced downregulation of HGF release. Using three-dimensional culture models of angiogenesis, we demonstrated that VEGF and HGF were both responsible for the high angiogenic potential of SHED through direct targeting of endothelial cells. In addition, FGF-2 treatment increased the fraction of Stro-1+/CD146+ progenitor cells. We then applied in vitro FGF-2 priming to SHED before encapsulation in hydrogels and in vivo subcutaneous implantation. Our results showed that FGF-2 priming is more efficient than hypoxia at increasing SHED-induced vascularization compared with nonprimed controls. Altogether, these data demonstrate that FGF-2 priming enhances the angiogenic potential of SHED through the secretion of both HGF and VEGF.

Indexed as

Cell HypoxiaDental PulpFibroblast Growth Factor 2Hepatocyte Growth FactorHumansMesenchymal Stem CellsMesenchymal Stem Cell TransplantationNeovascularization, PhysiologicTissue EngineeringVascular Endothelial Growth Factor AFibroblast Growth Factor 2Hepatocyte Growth FactorHGF protein, humanVascular Endothelial Growth Factor AAngiogenesisDynamic vascular imagingHepatocyte growth factorHypoxiaMesenchymal stem cellsPulp engineeringVascular endothelial growth factor

Identifiers

PMID26798059
PMCPMC4807665
OpenAlexW2262161474

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.