ArticleNature protocols2016
Isolation and cultivation of naive-like human pluripotent stem cells based on HERVH expression.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- De-extinction of the Northern white rhinoceros (Ceratotherium simum cottoni).The Journal of reproduction and development · 2026Review
- Co-option of an endogenous retrovirus (LTR7-HERVH) in early human embryogenesis: becoming useful and going unnoticed.Mobile DNA · 2025Review
- Developments and Applications of Liver-on-a-Chip Technology-Current Status and Future Prospects.Biomedicines · 2025Review
- Effect of a FOXO1 inhibitor on trophoblast differentiation from human pluripotent stem cells and ERV-associated gene expression.Regenerative therapy · 2024Article
- A new human embryonic cell type associated with activity of young transposable elements allows definition of the inner cell mass.PLoS biology · 2023Article
- Crotonylation of GAPDH regulates human embryonic stem cell endodermal lineage differentiation and metabolic switch.Stem cell research & therapy · 2023Article
- ZBTB12 is a molecular barrier to dedifferentiation in human pluripotent stem cells.Nature communications · 2023Article
- Zebrafish transposable elements show extensive diversification in age, genomic distribution, and developmental expression.Genome research · 2022Article
- Active endogenous retroviral elements in human pluripotent stem cells play a role in regulating host gene expression.Nucleic acids research · 2022Article
- Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction.Scientific reports · 2022Article
- The essential but enigmatic regulatory role of HERVH in pluripotency.Trends in genetics : TIG · 2022Review
- From Snapshots to Development: Identifying the Gaps in the Development of Stem Cell-based Embryo Models along the Embryonic Timeline.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- The Regulation and Functions of Endogenous Retrovirus in Embryo Development and Stem Cell Differentiation.Stem cells international · 2021Review
- Glutamine independence is a selectable feature of pluripotent stem cells.Nature metabolism · 2019Article
- Epigenetic reprogramming by naïve conditions establishes an irreversible state of partial X chromosome reactivation in female stem cells.Oncotarget · 2018Article
- Mapping human pluripotent stem cell differentiation pathways using high throughput single-cell RNA-sequencing.Genome biology · 2018Article
- Enigma of Retrotransposon Biology in Mammalian Early Embryos and Embryonic Stem Cells.Stem cells international · 2018Review
- Transcriptome variations among human embryonic stem cell lines are associated with their differentiation propensity.PloS one · 2018Article
- Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors.Journal of visualized experiments : JoVE · 2017Article
- Generation of an immortalized mesenchymal stem cell line producing a secreted biosensor protein for glucose monitoring.PloS one · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The ability to derive and stably maintain ground-state human pluripotent stem cells (hPSCs) that resemble the cells seen in vivo in the inner cell mass has the potential to be an invaluable tool for researchers developing stem cell-based therapies. To date, derivation of human naive-like pluripotent stem cell lines has been limited to a small number of lineages, and their long-term culturing remains problematic. We describe a protocol for genetic and phenotypic tagging, selecting and maintaining naive-like hPSCs. We tag hPSCs by GFP, expressed by the long terminal repeat (LTR7) of HERVH endogenous retrovirus. This simple and efficient protocol has been reproduced with multiple hPSC lines, including embryonic and induced pluripotent stem cells, and it takes ∼6 weeks. By using the reporter, homogeneous hPSC cultures can be derived, characterized and maintained for the long term by repeated re-sorting and re-plating steps. The HERVH-expressing cells have a similar, but nonidentical, expression pattern to other naive-like cells, suggesting that alternative pluripotent states might exist.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.