ArticleScientific reports2016
Characterization of Induced Pluripotent Stem Cell Microvesicle Genesis, Morphology and Pluripotent Content.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Impact of drying on yield and composition of extracellular vesicles from Centella asiatica.Scientific reports · 2026Article
- Pluripotent stem cell-derived extracellular vesicles: Cell type-dependent effect on tumorigenicity in cancer cell lines.Journal of cell communication and signaling · 2026Article
- Proteomic Signatures in Extracellular Vesicles as Predictive Markers of Neural and Cardiac Differentiation from Human iPSCs.Mass spectrometry (Tokyo, Japan) · 2026Article
- Differentiation of Human Adipose-derived Stem Cells to Exosome-affected Neural-like Cells Extracted from Human Cerebrospinal Fluid Using Bioprinting Process.Current stem cell research & therapy · 2024Article
- Bio-Inspired Nanocarriers Derived from Stem Cells and Their Extracellular Vesicles for Targeted Drug Delivery.Pharmaceutics · 2023Review
- Human pluripotent stem cell-derived extracellular vesicles: From now to the future.World journal of stem cells · 2023Review
- Nano-Messengers of the Heart: Promising Theranostic Candidates for Cardiovascular Maladies.Frontiers in physiology · 2022Review
- Epithelial-Cell-Derived Extracellular Vesicles in Pathophysiology of Epithelial Injury and Repair in Chronic Rhinosinusitis: Connecting Immunology in Research Lab to Biomarkers in Clinics.International journal of molecular sciences · 2021Review
- Neutrophil-derived trail is a proinflammatory subtype of neutrophil-derived extracellular vesicles.Theranostics · 2021Article
- Role of Stem Cell-Derived Microvesicles in Cardiovascular Disease.Journal of cardiovascular pharmacology · 2020Review
- Exosomes: Cell-Free Therapy for Cardiovascular Diseases.Journal of cardiovascular translational research · 2020Review
- Induced Pluripotent Stem Cells: Hope in the Treatment of Diseases, including Muscular Dystrophies.International journal of molecular sciences · 2020Review
- Human Pluripotent Stem Cell-Derived Extracellular Vesicles: Characteristics and Applications.Tissue engineering. Part B, Reviews · 2020Review
- Analysis of Adult Neural Retina Extracellular Vesicle Release, RNA Transport and Proteomic Cargo.Investigative ophthalmology & visual science · 2020Article
- Immunoregulatory Effects of Stem Cell-Derived Extracellular Vesicles on Immune Cells.Frontiers in immunology · 2020Review
- Article
- Considerations and Implications in the Purification of Extracellular Vesicles - A Cautionary Tale.Frontiers in neuroscience · 2019Article
- Exosomes in Acquired Neurological Disorders: New Insights into Pathophysiology and Treatment.Molecular neurobiology · 2018Review
- Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins.Scientific reports · 2018Article
- Flow virometry as a tool to study viruses.Methods (San Diego, Calif.) · 2018Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microvesicles (MVs) are lipid bilayer-covered cell fragments that range in diameter from 30 nm-1 uM and are released from all cell types. An increasing number of studies reveal that MVs contain microRNA, mRNA and protein that can be detected in the extracellular space. In this study, we characterized induced pluripotent stem cell (iPSC) MV genesis, content and fusion to retinal progenitor cells (RPCs) in vitro. Nanoparticle tracking revealed that iPSCs released approximately 2200 MVs cell/hour in the first 12 hrs with an average diameter of 122 nm. Electron and light microscopic analysis of iPSCs showed MV release via lipid bilayer budding. The mRNA content of iPSC MVs was characterized and revealed the presence of the transcription factors Oct-3/4, Nanog, Klf4, and C-Myc. The protein content of iPSCs MVs, detected by immunogold electron microscopy, revealed the presence of the Oct-3/4 and Nanog. Isolated iPSC MVs were shown to fuse with RPCs in vitro at multiple points along the plasma membrane. These findings demonstrate that the mRNA and protein cargo in iPSC MVs have established roles in maintenance of pluripotency. Building on this work, iPSC derived MVs may be shown to be involved in maintaining cellular pluripotency and may have application in regenerative strategies for neural tissue.
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