ArticleParasites & vectors2016
Characterization of hemolymph phenoloxidase activity in two Biomphalaria snail species and impact of Schistosoma mansoni infection.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- Limited impact of schistosome infection on Biomphalaria glabrata snail microbiomes.Parasites & vectors · 2026Article
- Computer-aided molecular and biological-immune modeling of illicium verum bioactive compounds employing the Egyptian Nile snail Biomphalaria alexandrina as a paradigm.Journal of computer-aided molecular design · 2025Article
- Effects of a supplemented diet containing 7 probiotic strains (Honeybeeotic) on honeybee physiology and immune response: analysis of hemolymph cytology, phenoloxidase activity, and gut microbiome.Biological research · 2024Article
- Impact of environmental factors on Biomphalaria pfeifferi vector capacity leading to human infection by Schistosoma mansoni in two regions of western Côte d'Ivoire.Parasites & vectors · 2024Article
- No evidence for schistosome parasite fitness trade-offs in the intermediate and definitive host.Parasites & vectors · 2023Article
- In search of the Aplysia immunome: an in silico study.BMC genomics · 2022Article
- Cellular and humoral immune response between snail hosts and their parasites.Frontiers in immunology · 2022Review
- Density-Dependent Prophylaxis in Freshwater Snails Driven by Oxylipin Chemical Cues.Frontiers in immunology · 2022Article
- Genetic architecture of transmission stage production and virulence in schistosome parasites.Virulence · 2021Article
- Different Immune Responses of the Lymphoid Organ in Shrimp at Early Challenge Stage ofAnimals : an open access journal from MDPI · 2021Article
- Transcriptome profiling of Lymnaea stagnalis (Gastropoda) for ecoimmunological research.BMC genomics · 2021Article
- Efficacy of Miltefosine and Artemether on InfectedHelminthologia · 2020Article
- Investigation of defense response and immune priming in Biomphalaria glabrata and Biomphalaria straminea, two species with different susceptibility to Schistosoma mansoni.Parasitology research · 2020Article
- Transcriptional responses of Biomphalaria pfeifferi and Schistosoma mansoni following exposure to niclosamide, with evidence for a synergistic effect on snails following exposure to both stressors.PLoS neglected tropical diseases · 2019Article
- Striking differences in virulence, transmission and sporocyst growth dynamics between two schistosome populations.Parasites & vectors · 2019Article
- The in vivo transcriptome of Schistosoma mansoni in the prominent vector species Biomphalaria pfeifferi with supporting observations from Biomphalaria glabrata.PLoS neglected tropical diseases · 2019Article
- Inbreeding does not alter the response to an experimental heat wave in a freshwater snail.PloS one · 2019Article
- The genome of the oyster Saccostrea offers insight into the environmental resilience of bivalves.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Relative importance of chemical attractiveness to parasites for susceptibility to trematode infection.Ecology and evolution · 2018Article
- Identification of a phenoloxidase- and melanin-dependent defence mechanism in Achatina fulica infected with Angiostrongylus vasorum.Parasites & vectors · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBiomphalaria snails are the intermediate host of the blood fluke Schistosoma mansoni, which infect more than 67 million people in tropical areas. Phenoloxidase enzymes (POs), including tyrosinases, catecholases, and laccases, are known to play a role in the immune defenses of arthropods, but the PO activity present in Biomphalaria spp. hemolymph has not been characterized. This study was designed to characterize substrate specificity and reaction optima of PO activity in Biomphalaria spp. hemolymph as a starting point to understand the role of this important invertebrate enzyme activity in snail biology and snail-schistosome interactions.
methodsWe used spectrophotometric assays with 3 specific substrates (L-tyrosine for tyrosinase, L-DOPA for catecholase, and PPD for laccase) and diethylthiocarbarmate (DETC) as specific PO inhibitor to characterize PO activity in the hemolymph of uninfected snails from two Biomphalaria species, and to determine the impact of the parasite Schistosoma mansoni on the PO activity of its B. glabrata vector.
resultsWe identified laccase activity in hemolymph from uninfected B. glabrata and B. alexandrina. For both species, the activity was optimal at 45 °C and pH 8.5, and located in the plasma. The K m and V max of PO enzymes are 1.45 mM and 0.024 OD.min(-1) for B. glabrata, and 1.19 mM and 0.025 OD.min(-1) for B. alexandrina. When the snail vector is parasitized by S. mansoni, we observed a sharp reduction in laccase activity seven weeks after snail infection.
conclusionsWe employed a highly specific spectrophotometric assay using PPD substrate which allows accurate measurement of laccase activity in Biomphalaria spp. hemolymph. We also demonstrated a strong impact of the parasite S. mansoni on laccase activity in the snail host.
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