Evidence map›Paper›PMID 26796138›Full record

ArticleScientific reports2016

Signal-off impedimetric immunosensor for the detection of Escherichia coli O157:H7.

Jingzhuan Wan, Junjie Ai, Yonghua Zhang, Xiaohui Geng, Qiang Gao, Zhiliang Cheng

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 72 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. FeBiosensors · 2023
    Article
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  7. Review
  8. Review
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  10. Electrochemical biosensors for pathogen detection.Biosensors & bioelectronics · 2020
    Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Jingzhuan WanKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
Junjie AiKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
Yonghua ZhangKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
Xiaohui GengKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
Qiang GaoKey Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, China.
Zhiliang ChengDepartment of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.
Shaanxi Normal University · CNUniversity of Pennsylvania · US

Funding

Tumor-targeted Polymersomes to image and Treat Ovarian CancerR01CA175480 · NCI · UNIVERSITY OF PENNSYLVANIA · PI CHENG, ZHILIANG · 2013 to 2017
$1.6M
NCI NIH HHS R01 CA175480NCI NIH HHS R01CA175480
6 · The paper itself

Abstract

A signal-off impedimetric immune-biosensor based on gold nanoparticle (AuNP)-mediated electron transfer (ET) across a self-assembled monolayer (SAM) was the developed for highly sensitive detection of Escherichia coli O157:H7 bacteria. The biosensor was fabricated by covalently grafting an anti-Escherichia coli O157:H7 antibody onto SAM-modified gold electrodes. Following bacterial capture, the sensor was further modified by the gold nanoparticles (AuNPs). Due to the strong interaction between AuNPs and Escherichia coli O157:H7, AuNPs attached to the surface of the bacteria and acted as ET pathways across the insulating SAMs on the electrode surface, resulting in a significant reduction of the electron transfer resistance (Ret) between the [Fe(CN)6](3-/4-) redox probe in the solution and the substrate gold surface. Therefore, the attachment AuNPs to captured bacteria significantly enhanced the sensitivity for Escherichia coli O157:H7 bacteria detection.

Indexed as

Biosensing TechniquesDielectric SpectroscopyEscherichia coli O157

Identifiers

PMID26796138
PMCPMC4726323
OpenAlexW2415472675

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.