Evidence map›Paper›PMID 26795621›Full record

ArticleClinical oral investigations2016

Effects of zoledronic acid and geranylgeraniol on the cellular behaviour and gene expression of primary human alveolar osteoblasts.

S Zafar, D E Coates, M P Cullinan, B K Drummond, T Milne, G J Seymour

Abstract read
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In one paragraph

Article in Clinical oral investigations, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 2 pooled it
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 43 citations in OpenAlex.

  1. Pooled it
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  12. StandardizedClinical, cosmetic and investigational dentistry · 2020
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  18. Wound healing protein profiles in the postoperative exudate of bisphosphonate-related osteonecrosis of mandible.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

S ZafarSir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 310 Great King Street, PO Box 647, Dunedin, 9054, New Zealand. sobia.zafar@uwa.edu.au.
D E CoatesSir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 310 Great King Street, PO Box 647, Dunedin, 9054, New Zealand.
M P CullinanSir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 310 Great King Street, PO Box 647, Dunedin, 9054, New Zealand.
B K DrummondSir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 310 Great King Street, PO Box 647, Dunedin, 9054, New Zealand.
T MilneSir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 310 Great King Street, PO Box 647, Dunedin, 9054, New Zealand.
G J SeymourSir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 310 Great King Street, PO Box 647, Dunedin, 9054, New Zealand.
University of Otago · NZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionBisphosphonate-related osteonecrosis of the jaw (BRONJ) is a serious complication of bisphosphonate therapy. The mechanism underlying BRONJ pathogenesis is poorly understood.

objectivesTo determine the effects of zoledronic acid (ZA) and geranylgeraniol (GGOH) on the mevalonate pathway (MVP) in osteoblasts generated from the human mandibular alveolar bone in terms of cell viability/proliferation, migration, apoptosis and gene expression. MATERIALS AND

methodsPrimary human osteoblasts (HOBs) isolated from the mandibular alveolar bone were phenotyped. HOBs were cultured with or without ZA and GGOH for up to 72 h. Cellular behaviour was examined using a CellTiter-Blue® viability assay, an Ibidi culture-insert migration assay, an Apo-ONE® Homogeneous Caspase-3/7 apoptosis assay and transmission electron microscopy (TEM). Quantitative real-time reverse transcriptase polymerase chain reaction (qRT

resultsZA decreased cell viability and migration and induced apoptosis in HOBs. TEM revealed signs of apoptosis in ZA-treated HOBs. However, the co-addition of GGOH ameliorated the effect of ZA and partially restored the cells to the control state. Twenty-eight genes in the osteogenic array and 27 genes in the angiogenic array were significantly regulated in the presence of ZA compared with those in the controls at one or more time points.

conclusionThe cytotoxic effect of ZA on HOBs and its reversal by the addition of GGOH suggests that the effect of ZA on HOBs is mediated via the MVP. CLINICAL RELEVANCE: The results suggest that GGOH could be used as a possible therapeutic/preventive strategy for BRONJ.

Indexed as

AdolescentAdultAlveolar ProcessApoptosisBiomarkersBone Density Conservation AgentsCell MovementCell ProliferationCells, CulturedCell SurvivalDiphosphonatesDiterpenesFemaleGene ExpressionHumansImidazolesBiomarkersBone Density Conservation AgentsDiphosphonatesDiterpenesgeranylgeraniolImidazolesZoledronic AcidAlveolar osteoblastsBRONJGeranylgeraniolMevalonate pathwayZoledronic acid

Identifiers

PMID26795621
OpenAlexW2284703075

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.