ArticlePLoS pathogens2016
Bacillus thuringiensis Crystal Protein Cry6Aa Triggers Caenorhabditis elegans Necrosis Pathway Mediated by Aspartic Protease (ASP-1).
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 76 citations in OpenAlex.
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- Potential Mechanisms Underlying the Minimal Impact of Cry1Ab1 Protein onInternational journal of molecular sciences · 2025Article
- A new understanding of Acanthamoeba castellanii: dispelling the role of bacterial pore-forming toxins in cyst formation and amoebicidal actions.Cell death discovery · 2025Article
- Resistance to Cry14A family Bacillus thuringiensis crystal proteins in Caenornabditis elegans operates via the nhr-31 transcription factor and vacuolar-type ATPase pathway.PLoS pathogens · 2024Article
- Neuro-intestinal acetylcholine signalling regulates the mitochondrial stress response in Caenorhabditis elegans.Nature communications · 2024Article
- 3-(Methylthio)Propionic Acid fromInternational journal of molecular sciences · 2024Article
- Aminopeptidase MNP-1 triggers intestine protease production by activating daf-16 nuclear location to degrade pore-forming toxins in Caenorhabditis elegans.PLoS pathogens · 2023Article
- A carnivorous mushroom paralyzes and kills nematodes via a volatile ketone.Science advances · 2023Article
- GPCR signaling regulates severe stress-induced organismic death in Caenorhabditis elegans.Aging cell · 2023Article
- Engineered pine endophyticFrontiers in microbiology · 2023Article
- Towards Understanding the Function of Aegerolysins.Toxins · 2022Review
- C. elegans monitor energy status via the AMPK pathway to trigger innate immune responses against bacterial pathogens.Communications biology · 2022Article
- Noncanonical necrosis in 2 different cell types in a Caenorhabditis elegans NAD+ salvage pathway mutant.G3 (Bethesda, Md.) · 2022Article
- Lysoptosis is an evolutionarily conserved cell death pathway moderated by intracellular serpins.Communications biology · 2022Article
- Identification of the Genes of the Plant PathogenFrontiers in microbiology · 2022Article
- Novel Nematode-Killing Protein-1 (Nkp-1) from a Marine Epiphytic BacteriumBiomedicines · 2021Article
- Article
- Cell Death and Transcriptional Responses Induced in Larvae of the NematodePharmaceuticals (Basel, Switzerland) · 2021Article
- Transcriptomic insights into the effects of CytCo, a novel nematotoxic protein, on the pine wood nematode Bursaphelenchus xylophilus.BMC genomics · 2021Article
- Pseudomonas aeruginosa cleaves the decoding center of Caenorhabditis elegans ribosomes.PLoS biology · 2020Article
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell death plays an important role in host-pathogen interactions. Crystal proteins (toxins) are essential components of Bacillus thuringiensis (Bt) biological pesticides because of their specific toxicity against insects and nematodes. However, the mode of action by which crystal toxins to induce cell death is not completely understood. Here we show that crystal toxin triggers cell death by necrosis signaling pathway using crystal toxin Cry6Aa-Caenorhabditis elegans toxin-host interaction system, which involves an increase in concentrations of cytoplasmic calcium, lysosomal lyses, uptake of propidium iodide, and burst of death fluorescence. We find that a deficiency in the necrosis pathway confers tolerance to Cry6Aa toxin. Intriguingly, the necrosis pathway is specifically triggered by Cry6Aa, not by Cry5Ba, whose amino acid sequence is different from that of Cry6Aa. Furthermore, Cry6Aa-induced necrosis pathway requires aspartic protease (ASP-1). In addition, ASP-1 protects Cry6Aa from over-degradation in C. elegans. This is the first demonstration that deficiency in necrosis pathway confers tolerance to Bt crystal protein, and that Cry6A triggers necrosis represents a newly added necrosis paradigm in the C. elegans. Understanding this model could lead to new strategies for nematode control.
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