ArticleMethods in enzymology2016
Mechanical Properties of Intermediate Filament Proteins.
Article in Methods in enzymology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 85 citations in OpenAlex.
- Metal Ion Binding of Vimentin Tail Domain Fragments.Chembiochem : a European journal of chemical biology · 2026Article
- Keratin intermediate filaments mechanically position melanin pigments for genome photoprotection.Nature cell biology · 2026Article
- Vimentin - Force regulator in confined environments.Current opinion in cell biology · 2025Review
- Engineered Recombinant Hagfish Intermediate Filament Proteins: Unraveling Domain Roles in Synthetic Fiber Formation and Mechanics.ACS omega · 2024Article
- KCNK1 promotes proliferation and metastasis of breast cancer cells by activating lactate dehydrogenase A (LDHA) and up-regulating H3K18 lactylation.PLoS biology · 2024Article
- Vimentin is a key regulator of cell mechanosensing through opposite actions on actomyosin and microtubule networks.Communications biology · 2024Article
- A fusion protein of vimentin with Fc fragment inhibits Japanese encephalitis virus replication.Frontiers in veterinary science · 2024Article
- The laminin-keratin link shields the nucleus from mechanical deformation and signalling.Nature materials · 2023Article
- Proteomic interrogation of the meninges reveals the molecular identities of structural components and regional distinctions along the CNS axis.Fluids and barriers of the CNS · 2023Article
- Retinal gliosis and phenotypic diversity of intermediate filament induction and remodeling upon acoustic blast overpressure (ABO) exposure to the rat eye.Experimental eye research · 2023Article
- From the membrane to the nucleus: mechanical signals and transcription regulation.Biophysical reviews · 2023Review
- Membrane-Bound Vimentin Filaments Reorganize and Elongate under Strain.Biomacromolecules · 2023Article
- Mechanobiology and survival strategies of circulating tumor cells: a process towards the invasive and metastatic phenotype.Frontiers in cell and developmental biology · 2023Review
- Effects of vimentin on the migration, search efficiency, and mechanical resilience of dendritic cells.Biophysical journal · 2022Article
- Spring-like behavior of cytoplasm holds the mitotic spindle in place.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Multisensory Network Drives Nuclear Mechanoadaptation.Biomolecules · 2022Review
- The role of physical cues in the development of stem cell-derived organoids.European biophysics journal : EBJ · 2022Review
- Vimentin Intermediate Filaments Mediate Cell Morphology on Viscoelastic Substrates.ACS applied bio materials · 2022Article
- Molecular, morphological and functional properties of tunnelling nanotubes between normal and cancer urothelial cells: New insights from theFrontiers in cell and developmental biology · 2022Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Purified intermediate filament (IF) proteins can be reassembled in vitro to produce polymers closely resembling those found in cells, and these filaments form viscoelastic gels. The cross-links holding IFs together in the network include specific bonds between polypeptides extending from the filament surface and ionic interactions mediated by divalent cations. IF networks exhibit striking nonlinear elasticity with stiffness, as quantified by shear modulus, increasing an order of magnitude as the networks are deformed to large strains resembling those that soft tissues undergo in vivo. Individual IFs can be stretched to more than two or three times their resting length without breaking. At least 10 different rheometric methods have been used to quantify the viscoelasticity of IF networks over a wide range of timescales and strain magnitudes. The mechanical roles of different classes of cytoplasmic IFs on mesenchymal and epithelial cells in culture have also been studied by an even wider range of microrheological methods. These studies have documented the effects on cell mechanics when IFs are genetically or pharmacologically disrupted or when normal or mutant IF proteins are exogenously expressed in cells. Consistent with in vitro rheology, the mechanical role of IFs is more apparent as cells are subjected to larger and more frequent deformations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.