ArticleScientific reports2016
Photoactivated adenylyl cyclase (PAC) reveals novel mechanisms underlying cAMP-dependent axonal morphogenesis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Unveiling the Photoactivation Mechanism of BLUF Photoreceptor Protein through Hybrid Quantum Mechanics/Molecular Mechanics Free-Energy Calculation.ACS physical chemistry Au · 2024Article
- All-optical presynaptic plasticity induction by photoactivated adenylyl cyclase targeted to axon terminals.Cell reports methods · 2024Article
- Optogenetic demonstration of the involvement of SMA-negative mural cells in the regulation of cerebral blood flow.Frontiers in physiology · 2023Article
- NPC transplantation rescues sci-driven cAMP/EPAC2 alterations, leading to neuroprotection and microglial modulation.Cellular and molecular life sciences : CMLS · 2022Article
- Multifaceted Roles of cAMP Signaling in the Repair Process of Spinal Cord Injury and Related Combination Treatments.Frontiers in molecular neuroscience · 2022Review
- Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV.Cell death discovery · 2021Article
- Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases.RSC chemical biology · 2021Article
- Astrocytic cAMP modulates memory via synaptic plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Molecular Properties and Optogenetic Applications of Enzymerhodopsins.Advances in experimental medicine and biology · 2021Review
- EPAC2: A new and promising protein for glioma pathogenesis and therapy.Oncology reviews · 2020Article
- Optogenetic Manipulation of Postsynaptic cAMP Using a Novel Transgenic Mouse Line Enables Synaptic Plasticity and Enhances Depolarization Following Tetanic Stimulation in the Hippocampal Dentate Gyrus.Frontiers in neural circuits · 2020Article
- Optogenetic Techniques for Manipulating and Sensing G Protein-Coupled Receptor Signaling.Methods in molecular biology (Clifton, N.J.) · 2020Review
- The C-terminal region affects the activity of photoactivated adenylyl cyclase from Oscillatoria acuminata.Scientific reports · 2019Article
- Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases.Cell chemical biology · 2019Article
- The Astrocytic cAMP Pathway in Health and Disease.International journal of molecular sciences · 2019Review
- Phrenic motor neuron adenosine 2A receptors elicit phrenic motor facilitation.The Journal of physiology · 2018Article
- Intracellular cAMP Sensor EPAC: Physiology, Pathophysiology, and Therapeutics Development.Physiological reviews · 2018Review
- The Molecular and Cellular Mechanisms of Axon Guidance in Mossy Fiber Sprouting.Frontiers in neurology · 2018Review
- It takes a village to raise a branch: Cellular mechanisms of the initiation of axon collateral branches.Molecular and cellular neurosciences · 2017Review
- Modulation of cyclic nucleotide-mediated cellular signaling and gene expression using photoactivated adenylyl cyclase as an optogenetic tool.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatiotemporal regulation of axonal branching and elongation is essential in the development of refined neural circuits. cAMP is a key regulator of axonal growth; however, whether and how intracellular cAMP regulates axonal branching and elongation remain unclear, mainly because tools to spatiotemporally manipulate intracellular cAMP levels have been lacking. To overcome this issue, we utilized photoactivated adenylyl cyclase (PAC), which produces cAMP in response to blue-light exposure. In primary cultures of dentate granule cells transfected with PAC, short-term elevation of intracellular cAMP levels induced axonal branching but not elongation, whereas long-term cAMP elevation induced both axonal branching and elongation. The temporal dynamics of intracellular cAMP levels regulated axonal branching and elongation through the activation of protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac), respectively. Thus, using PAC, our study for the first time reveals that temporal cAMP dynamics could regulate axonal branching and elongation via different signaling pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.