ArticleScientific reports2016
Comparative proteomics reveals abnormal binding of ATGL and dysferlin on lipid droplets from pressure overload-induced dysfunctional rat hearts.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- A novel mitochondrial microprotein reprograms cellular bioenergetics and protects against hepatic steatosis.iScience · 2026Article
- Review
- Decoding the Lipid Droplet Proteome: New Frontiers in Cardiovascular Disease Research.International journal of molecular sciences · 2025Review
- Phosphatidic acid drives spatiotemporal distribution of Pex30 at ER-LD contact sites.The Journal of cell biology · 2025Article
- Tools to Dissect Lipid Droplet Regulation, Players, and Mechanisms.ACS chemical biology · 2025Review
- Bioengineered Model of Human LGMD2B Skeletal Muscle Reveals Roles of Intracellular Calcium Overload in Contractile and Metabolic Dysfunction in Dysferlinopathy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Measurement of ATGL activity using adiposomes.Biophysics reports · 2023Article
- Lipid Droplets: A Cellular Organelle Vital for Thermogenesis.International journal of biological sciences · 2022Review
- A Decade of Mighty Lipophagy: What We Know and What Facts We Need to Know?Oxidative medicine and cellular longevity · 2021Review
- Two Types of Contact Between Lipid Droplets and Mitochondria.Frontiers in cell and developmental biology · 2020Review
- Lipid droplets and mitochondria are anchored during brown adipocyte differentiation.Protein & cell · 2019Article
- Dysferlin deficiency alters lipid metabolism and remodels the skeletal muscle lipidome in mice.Journal of lipid research · 2019Article
- Review
- In vitro inhibition of hepatic stellate cell activation by the autophagy-related lipid droplet protein ATG2A.Scientific reports · 2018Article
- iTRAQ analysis of a mouse acute myocardial infarction model reveals that vitamin D binding protein promotes cardiomyocyte apoptosis after hypoxia.Oncotarget · 2018Article
- Proteomics analysis and proteogenomic characterization of different physiopathological human lenses.BMC ophthalmology · 2017Article
- Breaking fat: The regulation and mechanisms of lipophagy.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Dgat1 and Dgat2 regulate enterocyte triacylglycerol distribution and alter proteins associated with cytoplasmic lipid droplets in response to dietary fat.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
- B-1 cells modulate the murine macrophage response toWorld journal of biological chemistry · 2017Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Excessive retention of neutral lipids in cardiac lipid droplets (LDs) is a common observation in cardiomyopathy. Thus, the systematic investigation of the cardiac LD proteome will help to dissect the underlying mechanisms linking cardiac steatosis and myocardial dysfunction. Here, after isolation of LDs from normal and dysfunctional Sprague-Dawley rat hearts, we identified 752 heart-associated LD proteins using iTRAQ quantitative proteomic method, including 451 proteins previously unreported on LDs. The most noteworthy finding was the identification of the membrane resealing protein, dysferlin. An analysis of dysferlin truncation mutants indicated that its C2 domain was responsible for its LD localization. Quantitative proteomic results further determined that 27 proteins were increased and 16 proteins were decreased in LDs from post pressure overload-induced dysfunctional hearts, compared with normal hearts. Notably, adipose triacylglycerol lipase (ATGL) was dramatically decreased and dysferlin was substantially increased on dysfunctional cardiac LDs. This study for the first time reveals the dataset of the heart LD proteome in healthy tissue and the variation of it under cardiac dysfunction. These findings highlight an association between the altered LD protein localization of dysferlin and ATGL and myocardial dysfunction.
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