ArticleCytotherapy2016
Collagen Type II enhances chondrogenic differentiation in agarose-based modular microtissues.
Article in Cytotherapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 2 syntheses or guidelines pooled it, 75 citations in OpenAlex.
- Efficacy of decellularized extracellular matrix (dECM) for articular cartilage repair in osteoarthritis (OA): a systematic review and meta-analysis.Journal of orthopaedic surgery and research · 2025Pooled it
- Pooled it
- Injectable microsphere-based delivery strategies for stem cells and their derivatives in tissue regeneration.Bioactive materials · 2026Review
- 3D-bioprinting for joint regeneration.Frontiers in bioengineering and biotechnology · 2026Review
- In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones.Bioactive materials · 2025Article
- Hydrogel-Based 3D Bioprinting Technology for Articular Cartilage Regenerative Engineering.Gels (Basel, Switzerland) · 2024Review
- Type II collagen scaffolds repair critical-sized osteochondral defects under induced conditions of osteoarthritis in rat knee joints via inhibiting TGF-β-Smad1/5/8 signaling pathway.Bioactive materials · 2024Article
- Effect of amniotic membrane/collagen scaffolds on laryngeal cartilage repair.Laryngoscope investigative otolaryngology · 2024Article
- Collagen Type II-Based Injectable Materials for In situ Repair and Regeneration of Articular Cartilage Defect.Biomaterials research · 2024Article
- Investigating the Interplay Between Matrix Compliance and Passaging History on Chondrogenic Differentiation of Mesenchymal Stem Cells Encapsulated Within Alginate-Gelatin Hybrid Hydrogels.Annals of biomedical engineering · 2023Article
- Ion elemental-optimized layered double hydroxide nanoparticles promote chondrogenic differentiation and intervertebral disc regeneration of mesenchymal stem cells through focal adhesion signaling pathway.Bioactive materials · 2023Article
- Chondral/Desmal Osteogenesis in 3D Spheroids Sensitized by Psychostimulants.Journal of clinical medicine · 2022Article
- Decellularised Cartilage ECM Culture Coatings Drive Rapid and Robust Chondrogenic Differentiation of Human Periosteal Cells.Bioengineering (Basel, Switzerland) · 2022Article
- Functional tissue-engineered microtissue formed by self-aggregation of cells for peripheral nerve regeneration.Stem cell research & therapy · 2022Article
- Advances in Barrier Membranes for Guided Bone Regeneration Techniques.Frontiers in bioengineering and biotechnology · 2022Review
- Collagen type II: From biosynthesis to advanced biomaterials for cartilage engineering.Biomaterials and biosystems · 2021Review
- Native Collagen II Relieves Bone Impairment through Improving Inflammation and Oxidative Stress in Ageing db/db Mice.Molecules (Basel, Switzerland) · 2021Article
- A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type.Life (Basel, Switzerland) · 2021Article
- Core-binding factor beta is required for osteoblast differentiation during fibula fracture healing.Journal of orthopaedic surgery and research · 2021Article
- Potential and recent advances of microcarriers in repairing cartilage defects.Journal of orthopaedic translation · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
BACKGROUND
aimsCell-based therapies have made an impact on the treatment of osteoarthritis; however, the repair and regeneration of thick cartilage defects is an important and growing clinical problem. Next-generation therapies that combine cells with biomaterials may provide improved outcomes. We have developed modular microenvironments that mimic the composition of articular cartilage as a delivery system for consistently differentiated cells.
methodsHuman bone marrow-derived mesenchymal stem cells (MSC) were embedded in modular microbeads consisting of agarose (AG) supplemented with 0%, 10% and 20% collagen Type II (COL-II) using a water-in-oil emulsion technique. AG and AG/COL-II microbeads were characterized in terms of their structural integrity, size distribution and protein content. The viability of embedded MSC and their ability to differentiate into osteogenic, adipogenic and chondrogenic lineages over 3 weeks in culture were also assessed.
resultsMicrobeads made with <20% COL-II were robust, generally spheroidal in shape and 80 ± 10 µm in diameter. MSC viability in microbeads was consistently high over a week in culture, whereas viability in corresponding bulk hydrogels decreased with increasing COL-II content. Osteogenic differentiation of MSC was modestly supported in both AG and AG/COL-II microbeads, whereas adipogenic differentiation was strongly inhibited in COL-II containing microbeads. Chondrogenic differentiation of MSC was clearly promoted in microbeads containing COL-II, compared with pure AG matrices.
conclusionsInclusion of collagen Type II in agarose matrices in microbead format can potentiate chondrogenic differentiation of human MSC. Such compositionally tailored microtissues may find utility for cell delivery in next-generation cartilage repair therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.