ArticleMethods in cell biology2016
Axonal actin in action: Imaging actin dynamics in neurons.
Article in Methods in cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- A PDZ-RapGEF promotes synaptic development in Caenorhabditis elegans through a Rap/Rac signaling pathway.Development (Cambridge, England) · 2025Article
- Flow-Induced Shear Stress Combined with Microtopography Inhibits the Differentiation of Neuro-2a Cells.Micromachines · 2025Article
- Neurons: The Interplay between Cytoskeleton, Ion Channels/Transporters and Mitochondria.Cells · 2022Review
- Quantitative proteomics and in-cell cross-linking reveal cellular reorganisation during early neuronal differentiation of SH-SY5Y cells.Communications biology · 2022Article
- Contractile apparatus in CNS capillary pericytes.Neurophotonics · 2022Review
- Imaging Dendritic Spines in Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2021Article
- The Significant Role of the Microfilament System in Tumors.Frontiers in oncology · 2021Review
- Processive flow by biased polymerization mediates the slow axonal transport of actin.The Journal of cell biology · 2019Article
- Nuclear and membrane estrogen receptor antagonists induce similar mTORC2 activation-reversible changes in synaptic protein expression and actin polymerization in the mouse hippocampus.CNS neuroscience & therapeutics · 2018Article
- Recent advances in branching mechanisms underlying neuronal morphogenesis.F1000Research · 2018Review
- Article
- The cell's self-generated "electrome": The biophysical essence of the immaterial dimension of Life?Communicative & integrative biology · 2016Article
- A dynamic formin-dependent deep F-actin network in axons.The Journal of cell biology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Actin is a highly conserved, key cytoskeletal protein involved in numerous structural and functional roles. In neurons, actin has been intensively investigated in axon terminals-growth cones-and dendritic spines, but details about actin structure and dynamics in axon shafts have remained obscure for decades. A major barrier in the field has been imaging actin. Actin exists as soluble monomers (G-actin) as well as actin filaments (F-actin), and labeling actin with conventional fluorescent probes like GFP/RFP typically leads to a diffuse haze that makes it difficult to discern kinetic behaviors. In a recent publication, we used F-actin selective probes to visualize actin dynamics in axons, resolving striking actin behaviors that have not been described before. However, using these probes to visualize actin dynamics is challenging as they can cause bundling of actin filaments; thus, experimental parameters need to be strictly optimized. Here we describe some practical methodological details related to using these probes for visualizing F-actin dynamics in axons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.