Evidence map›Paper›PMID 26794208›Full record

ArticleLangmuir : the ACS journal of surfaces and colloids2016

Photopolymerization of Dienoyl Lipids Creates Planar Supported Poly(lipid) Membranes with Retained Fluidity.

Kristina S Orosz, Ian W Jones, John P Keogh, Christopher M Smith, Kaitlyn R Griffin, Juhua Xu, Troy J Comi, H K Hall, S Scott Saavedra

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Langmuir : the ACS journal of surfaces and colloids, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Kristina S OroszDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Ian W JonesDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
John P KeoghDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Christopher M SmithDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Kaitlyn R GriffinDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Juhua XuDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
Troy J ComiDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
H K HallDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
S Scott SaavedraDepartment of Chemistry and Biochemistry and ‡BIO5 Institute, University of Arizona , 1306 East University Boulevard, Tucson, Arizona 85721, United States.
University of Arizona · US

Funding

Training Program in Chemistry-Interface with BiologyT32GM070421 · NIGMS · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI HERGENROTHER, PAUL · 2005 to 2019
$5.4M
Sniffer Biosensors Based on Ion Channel-GPCR Chimeras and Polylipid MembranesR01EB007047 · NIBIB · UNIVERSITY OF ARIZONA · PI ASPINWALL, CRAIG A, SAAVEDRA, STEVEN SCOTT · 2006 to 2015
$3.5M
NIBIB NIH HHS R01 EB007047NIBIB NIH HHS R01EB007047NIGMS NIH HHS T32 GM070421
6 · The paper itself

Abstract

Polymerization of substrate-supported bilayers composed of dienoylphosphatidylcholine (PC) lipids is known to greatly enhance their chemical and mechanical stability; however, the effects of polymerization on membrane fluidity have not been investigated. Here planar supported lipid bilayers (PSLBs) composed of dienoyl PCs on glass substrates were examined to assess the degree to which UV-initiated polymerization affects lateral lipid mobility. Fluorescence recovery after photobleaching (FRAP) was used to measure the diffusion coefficients (D) and mobile fractions of rhodamine-DOPE in unpolymerized and polymerized PSLBs composed of bis-sorbyl phosphatidylcholine (bis-SorbPC), mono-sorbyl-phosphatidylcholine (mono-SorbPC), bis-dienoyl-phosphatidylcholine (bis-DenPC), and mono-dienoyl phosphatidylcholine (mono-DenPC). Polymerization was performed in both the Lα and Lβ phase for each lipid. In all cases, polymerization reduced membrane fluidity; however, measurable lateral diffusion was retained which is attributed to a low degree of polymerization. The D values for sorbyl lipids were less than those of the denoyl lipids; this may be a consequence of the distal location of polymerizable group in the sorbyl lipids which may facilitate interleaflet bonding. The D values measured after polymerization were 0.1-0.8 of those measured before polymerization, a range that corresponds to fluidity intermediate between that of a Lα phase and a Lβ phase. This D range is comparable to ratios of D values reported for liquid-disordered (Ld) and liquid-ordered (Lo) lipid phases and indicates that the effect of UV polymerization on lateral diffusion in a dienoyl PSLB is similar to the transition from a Ld phase to a Lo phase. The partial retention of fluidity in UV-polymerized PSLBs, their enhanced stability, and the activity of incorporated transmembrane proteins and peptides is discussed.

Indexed as

DiffusionFluorescence Recovery After PhotobleachingLipid BilayersMembrane FluidityPhosphatidylcholinesPhosphatidylethanolaminesPolymerizationRhodaminesTransition TemperatureUltraviolet Rays1,2-oleoylphosphatidylcholineLipid BilayersN-(lissamine rhodamine B sulfonyl)-1,2-dioleoylphosphatidylethanolaminePhosphatidylcholinesPhosphatidylethanolaminesRhodamines

Identifiers

PMID26794208
PMCPMC4755918
OpenAlexW2337913987

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.