ArticleDisease models & mechanisms2016
Multimodal analysis of ocular inflammation using the endotoxin-induced uveitis mouse model.
Article in Disease models & mechanisms, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 49 citations in OpenAlex.
- Microglia-independent rAAV-induced inflammation causes persistent ocular immune dysregulation rescued by S1P receptor modulation.Molecular therapy : the journal of the American Society of Gene Therapy · 2026Article
- Perivascular Macrophages Are Associated With Permissive Sites of Immune Cell Extravasation in Retinal Venules.Investigative ophthalmology & visual science · 2026Article
- IL-23 drives uveitis by acting on a population of tissue-resident entheseal T cells.JCI insight · 2025Article
- Autophagy Regulates Müller Glial Cell Inflammatory Activation.Investigative ophthalmology & visual science · 2025Article
- Article
- Photoreceptor loss does not recruit neutrophils despite strong microglial activation.bioRxiv : the preprint server for biology · 2025Article
- 3K3A-Activated Protein C Prevents Microglia Activation, Inhibits NLRP3 Inflammasome and Limits Ocular Inflammation.International journal of molecular sciences · 2022Article
- Recent advances in optical coherence tomography for anterior segment imaging in small animals and their clinical implications.The ocular surface · 2022Review
- Low-dose melittin is safe for intravitreal administration and ameliorates inflammation in an experimental model of uveitis.Current research in pharmacology and drug discovery · 2022Article
- Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis.Journal of visualized experiments : JoVE · 2021Article
- Myeloid Cells in the Mouse Retina and Uveal Tract Respond Differently to Systemic Inflammatory Stimuli.Investigative ophthalmology & visual science · 2021Article
- Ruxolitinib Alleviates Uveitis Caused byMicroorganisms · 2021Article
- Short chain fatty acids inhibit endotoxin-induced uveitis and inflammatory responses of retinal astrocytes.Experimental eye research · 2021Article
- Article
- Dynamics of Cyclooxygenase-1 Positive Microglia/Macrophage in the Retina of Pathological Model Mice as a Biomarker of the Retinal Inflammatory Diseases.International journal of molecular sciences · 2021Article
- Corneal Application of R9-SOCS1-KIR Peptide Alleviates Endotoxin-Induced Uveitis.Translational vision science & technology · 2021Article
- Engineering adeno-associated viral vectors to evade innate immune and inflammatory responses.Science translational medicine · 2021Article
- Characterization of Gene Therapy Associated Uveitis Following Intravitreal Adeno-Associated Virus Injection in Mice.Investigative ophthalmology & visual science · 2021Article
- Quantitative Assessment of Experimental Ocular Inflammatory Disease.Frontiers in immunology · 2021Review
- Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Endotoxin-induced uveitis (EIU) in rodents is a model of acute Toll-like receptor 4 (TLR4)-mediated organ inflammation, and has been used to model human anterior uveitis, examine leukocyte trafficking and test novel anti-inflammatory therapeutics. Wider adoption has been limited by the requirement for manual, non-specific, cell-count scoring of histological sections from each eye as a measure of disease severity. Here, we describe a comprehensive and efficient technique that uses ocular dissection and multimodal tissue analysis. This allows matched disease scoring by multicolour flow cytometric analysis of the inflammatory infiltrate, protein analysis on ocular supernatants and qPCR on remnant tissues of the same eye. Dynamic changes in cell populations could be identified and mapped to chemokine and cytokine changes over the course of the model. To validate the technique, dose-responsive suppression of leukocyte infiltration by recombinant interleukin-10 was demonstrated, as well as selective suppression of the monocyte (CD11b+Ly6C+) infiltrate, in mice deficient for eitherCcl2orCcr2 Optical coherence tomography (OCT) was used for the first time in this model to allowin vivoimaging of infiltrating vitreous cells, and correlated with CD11b+Ly6G+ counts to provide another unique measure of cell populations in the ocular tissue. Multimodal tissue analysis of EIU is proposed as a new standard to improve and broaden the application of this model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.