ArticlePeerJ2016
Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria.
Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
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- An electron transport complex required in the gut sensitizesProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Diversity, metabolic potential and global distribution of the anaerobic fermentative bacteria Phylum Candidatus Cloacimonadota.Environmental microbiome · 2025Article
- A widespread hydrogenase supports fermentative growth of gut bacteria in healthy people.Nature microbiology · 2025Article
- The RNF/NQR redox pumps: a versatile system for energy transduction in bacteria and archaea.Applied microbiology and biotechnology · 2025Review
- The oxidoreductase activity of Rnf balances redox cofactors during fermentation of glucose to propionate in Prevotella.Scientific reports · 2023Article
- Purification and structural characterization of the NaNature communications · 2022Article
- Rnf and Fix Have Specific Roles during Aerobic Nitrogen Fixation in Azotobacter vinelandii.Applied and environmental microbiology · 2022Article
- Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation.Frontiers in bioengineering and biotechnology · 2022Article
- Energy conservation under extreme energy limitation: the role of cytochromes and quinones in acetogenic bacteria.Extremophiles : life under extreme conditions · 2021Review
- Identifying and Engineering Bottlenecks of Autotrophic Isobutanol Formation in RecombinantFrontiers in bioengineering and biotechnology · 2021Article
- Energy Conservation in Fermentations of Anaerobic Bacteria.Frontiers in microbiology · 2021Review
- The Rnf complex is a NaCommunications biology · 2020Article
- Do reactive oxygen species or does oxygen itself confer obligate anaerobiosis? The case of Bacteroides thetaiotaomicron.Molecular microbiology · 2020Article
- Redox controls metabolic robustness in the gas-fermenting acetogenProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Energy conservation involving 2 respiratory circuits.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Revealing the metabolic capacity ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Rnf Complex Is an Energy-Coupled Transhydrogenase Essential To Reversibly Link Cellular NADH and Ferredoxin Pools in the Acetogen Acetobacterium woodii.Journal of bacteriology · 2018Article
- Endogenous superoxide is a key effector of the oxygen sensitivity of a model obligate anaerobe.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A ferredoxin:NAD(+) oxidoreductase was recently discovered as a redox-driven ion pump in the anaerobic, acetogenic bacterium Acetobacterium woodii. The enzyme is assumed to be encoded by the rnf genes. Since these genes are present in the genomes of many bacteria, we tested for ferredoxin:NAD(+) oxidoreductase activity in cytoplasmic membranes from several different Gram-positive and Gram-negative bacteria that have annotated rnf genes. We found this activity in Clostridium tetanomorphum, Clostridium ljungdahlii, Bacteroides fragilis, and Vibrio cholerae but not in Escherichia coli and Rhodobacter capsulatus. As in A. woodii, the activity was Na(+)-dependent in C. tetanomorphum and B. fragilis but Na(+)-independent in C. ljungdahlii and V. cholerae. We deleted the rnf genes from B. fragilis and demonstrated that the mutant has greatly reduced ferredoxin:NAD(+) oxidoreductase activity. This is the first genetic proof that the rnf genes indeed encode the reduced ferredoxin:NAD(+) oxidoreductase activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.