Evidence map›Paper›PMID 26793181›Full record

ArticleFrontiers in microbiology2015

A Novel Loop-Mediated Isothermal Amplification Assay for Serogroup Identification of Neisseria meningitidis in Cerebrospinal Fluid.

DoKyung Lee, Eun Jin Kim, Paul E Kilgore, Hideyuki Takahashi, Makoto Ohnishi, Jun Tomono, Shigehiko Miyamoto, Daisuke Omagari, Dong Wook Kim, Mitsuko Seki

Abstract read
In one paragraph

Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Development of a rapid, simple multiplex PCR-dipstick assay for the detection ofFrontiers in cellular and infection microbiology · 2025
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  19. Laboratory Diagnosis of Central Nervous System Infection.Current infectious disease reports · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

DoKyung LeeDepartment of Pharmacy, College of Pharmacy, Hanyang UniversityAnsan, South Korea; Institute of Pharmacological Research, Hanyang UniversityAnsan, South Korea.
Eun Jin KimDepartment of Pharmacy, College of Pharmacy, Hanyang UniversityAnsan, South Korea; Institute of Pharmacological Research, Hanyang UniversityAnsan, South Korea.
Paul E KilgoreDepartment of Pharmacy Practice, Eugene Applebaum College of Pharmacy & Health Sciences, Wayne State University Detroit, MI, USA.
Hideyuki TakahashiDepartment of Bacteriology I, National Institute of Infectious Diseases Tokyo, Japan.
Makoto OhnishiDepartment of Bacteriology I, National Institute of Infectious Diseases Tokyo, Japan.
Jun TomonoKaneka, Co., Ltd., Osaka Japan.
Shigehiko MiyamotoKaneka, Co., Ltd., Osaka Japan.
Daisuke OmagariNihon University School of DentistryTokyo, Japan; Dental Research Center, Nihon University School of DentistryTokyo, Japan.
Dong Wook KimDepartment of Pharmacy, College of Pharmacy, Hanyang UniversityAnsan, South Korea; Institute of Pharmacological Research, Hanyang UniversityAnsan, South Korea.
Mitsuko SekiNihon University School of DentistryTokyo, Japan; Dental Research Center, Nihon University School of DentistryTokyo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have developed a novel Neisseria meningitidis serogroup-specific loop-mediated isothermal amplification (LAMP) assay for six of the most common meningococcal serogroups (A, B, C, W, X, and Y). The assay was evaluated using a set of 31 meningococcal LAMP assay positive cerebrospinal fluid (CSF) specimens from 1574 children with suspected meningitis identified in prospective surveillance between 1998 and 2002 in Vietnam, China, and Korea. Primer specificity was validated using 15 N. meningitidis strains (including serogroups A, B, C, E, W, X, Y, and Z) and 19 non-N. meningitidis species. The N. meningitidis serogroup LAMP detected down to ten copies and 100 colony-forming units per reaction. Twenty-nine CSF had N. meningitidis serogroup identified by LAMP compared with two CSF in which N. meningitidis serogroup was identified by culture and multi-locus sequence typing. This is the first report of a serogroup-specific identification assay for N. meningitidis using the LAMP method. Our results suggest that this assay will be a rapid, sensitive, and uniquely serogroup-specific assay with potential for application in clinical laboratories and public health surveillance systems.

Indexed as

cerebrospinal fluidchildrenloop-mediated isothermal amplificationmeningitisNeisseria meningitidisserogroup identification

Identifiers

PMID26793181
PMCPMC4709847

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.