ArticleFrontiers in microbiology2015
A Novel Loop-Mediated Isothermal Amplification Assay for Serogroup Identification of Neisseria meningitidis in Cerebrospinal Fluid.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- A pooled analysis of the LAMP assay for the detection of Neisseria meningitidis.BMC infectious diseases · 2020Pooled it
- A systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in the diagnosis of invasive meningococcal disease in children.BMC pediatrics · 2019Pooled it
- Design of New Primer Sets for the Development of a Loop-Mediated Isothermal Amplification for Rapid Detection ofCurrent issues in molecular biology · 2025Article
- Development of a rapid, simple multiplex PCR-dipstick assay for the detection ofFrontiers in cellular and infection microbiology · 2025Article
- Loop-Mediated Isothermal Amplification (LAMP): Potential Point-of-Care Testing for Vulvovaginal Candidiasis.Journal of fungi (Basel, Switzerland) · 2023Article
- LAMP assays for the simple and rapid detection of clinically important urinary pathogens including the detection of resistance to 3rd generation cephalosporins.BMC infectious diseases · 2021Article
- Elucidating the Structural and Minimal Protective Epitope of the Serogroup X Meningococcal Capsular Polysaccharide.Frontiers in molecular biosciences · 2021Article
- Article
- Molecular serotype-specific identification of Streptococcus pneumoniae using loop-mediated isothermal amplification.Scientific reports · 2019Article
- Development of a Novel Loop-Mediated Isothermal Amplification Method to Detect Guiana Extended-Spectrum (GES) β-Lactamase Genes inFrontiers in microbiology · 2019Article
- Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay to detectInfection and drug resistance · 2019Article
- A protocol for a systematic review of the diagnostic accuracy of Loop-mediated-isothermal AMPlification (LAMP) in diagnosis of invasive meningococcal disease in children.Systematic reviews · 2018Article
- An innovative diagnostic technology for the codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer.Malaria journal · 2018Article
- Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.Journal of applied microbiology · 2018Review
- Evaluation of an Internally Controlled Multiplex Tth Endonuclease Cleavage Loop-Mediated Isothermal Amplification (TEC-LAMP) Assay for the Detection of Bacterial Meningitis Pathogens.International journal of molecular sciences · 2018Article
- Loop-Mediated Isothermal Amplification Methods for Diagnosis of Bacterial Meningitis.Frontiers in pediatrics · 2018Review
- Stem loop-mediated isothermal amplification test: comparative analysis with classical LAMP and PCR in detection of Entamoeba histolytica in Kenya.BMC research notes · 2017Article
- Molecular Serotype-Specific Identification of Non-type bFrontiers in microbiology · 2017Article
- Laboratory Diagnosis of Central Nervous System Infection.Current infectious disease reports · 2016Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a novel Neisseria meningitidis serogroup-specific loop-mediated isothermal amplification (LAMP) assay for six of the most common meningococcal serogroups (A, B, C, W, X, and Y). The assay was evaluated using a set of 31 meningococcal LAMP assay positive cerebrospinal fluid (CSF) specimens from 1574 children with suspected meningitis identified in prospective surveillance between 1998 and 2002 in Vietnam, China, and Korea. Primer specificity was validated using 15 N. meningitidis strains (including serogroups A, B, C, E, W, X, Y, and Z) and 19 non-N. meningitidis species. The N. meningitidis serogroup LAMP detected down to ten copies and 100 colony-forming units per reaction. Twenty-nine CSF had N. meningitidis serogroup identified by LAMP compared with two CSF in which N. meningitidis serogroup was identified by culture and multi-locus sequence typing. This is the first report of a serogroup-specific identification assay for N. meningitidis using the LAMP method. Our results suggest that this assay will be a rapid, sensitive, and uniquely serogroup-specific assay with potential for application in clinical laboratories and public health surveillance systems.
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