ArticleToxicology and applied pharmacology2016
Genistein exposure inhibits growth and alters steroidogenesis in adult mouse antral follicles.
Article in Toxicology and applied pharmacology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Exposure to Long- and Short-Chain Per- and Polyfluoroalkyl Substances in Mice and Ovarian-Related Outcomes: AnEnvironmental health perspectives · 2025Article
- Mechanisms by which phytogenic extracts enhance livestock reproductive health: current insights and future directions.Frontiers in veterinary science · 2025Review
- The Therapeutic Role of Genistein in Perimenopausal and Postmenopausal Women.The Journal of clinical and aesthetic dermatology · 2024Review
- The Antioxidant Activity of Atomized Extracts of the Leaves and Stems ofMolecules (Basel, Switzerland) · 2024Article
- Article
- In vitro growth of the ovarian follicle: taking stock of advances in research.JBRA assisted reproduction · 2022Review
- Enhancement of Productive Performance, Bone Physical Characteristics, and Mineralization of Laying Hens during the Post-Peak Period by Genistein.Archives of Razi Institute · 2021Article
- Effects of Chronic Dietary Exposure to Phytoestrogen Genistein on Uterine Morphology in Mice.Journal of agricultural and food chemistry · 2021Article
- In vitro ovarian follicle growth: a comprehensive analysis of key protocol variables†.Biology of reproduction · 2020Review
- Oral Exposure to Genistein during Conception and Lactation Period Affects the Testicular Development of Male Offspring Mice.Animals : an open access journal from MDPI · 2020Article
- Genistein improves the reproductive performance and bone status of breeder hens during the late egg-laying period.Poultry science · 2019Article
- Genistein inhibits proliferation and induces senescence in neonatal mouse pituitary gland explant cultures.Toxicology · 2019Article
- The effects of dietary levels of genistein on ovarian follicle number and gene expression.Reproductive toxicology (Elmsford, N.Y.) · 2018Article
- Effects ofChinese medical journal · 2018Article
- Preconception exposure to dietary levels of genistein affects female reproductive outcomes.Reproductive toxicology (Elmsford, N.Y.) · 2017Article
- Doxorubicin Has Dose-Dependent Toxicity on Mouse Ovarian Follicle Development, Hormone Secretion, and Oocyte Maturation.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- Effects of isoliquiritigenin on ovarian antral follicle growth and steroidogenesis.Reproductive toxicology (Elmsford, N.Y.) · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Genistein is a naturally occurring isoflavone phytoestrogen commonly found in plant products such as soybeans, lentils, and chickpeas. Genistein, like other phytoestrogens, has the potential to mimic, enhance, or impair the estradiol biosynthesis pathway, thereby potentially altering ovarian follicle growth. Previous studies have inconsistently indicated that genistein exposure may alter granulosa cell proliferation and hormone production, but no studies have examined the effects of genistein on intact antral follicles. Thus, this study was designed to test the hypothesis that genistein exposure inhibits follicle growth and steroidogenesis in intact antral follicles. To test this hypothesis, antral follicles isolated from CD-1 mice were cultured with vehicle (dimethyl sulfoxide; DMSO) or genistein (6.0 and 36μM) for 18-96h. Every 24h, follicle diameters were measured to assess growth. At the end of each culture period, the media were pooled to measure hormone levels, and the cultured follicles were collected to measure expression of cell cycle regulators and steroidogenic enzymes. The results indicate that genistein (36μM) inhibits growth of mouse antral follicles. Additionally, genistein (6.0 and 36μM) increases progesterone, testosterone, and dehydroepiandrosterone (DHEA) levels, but decreases estrone and estradiol levels. The results also indicate that genistein alters the expression of steroidogenic enzymes at 24, 72 and 96h, and the expression of cell cycle regulators at 18h. These data indicate that genistein exposure inhibits antral follicle growth by inhibiting the cell cycle, alters sex steroid hormone levels, and dysregulates steroidogenic enzymes in cultured mouse antral follicles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.