Evidence map›Paper›PMID 26791443›Full record

ArticleElectrophoresis2016

Quantitative analysis of serotonin secreted by human embryonic stem cells-derived serotonergic neurons via pH-mediated online stacking-CE-ESI-MRM.

Xuefei Zhong, Ling Hao, Jianfeng Lu, Hui Ye, Su-Chun Zhang, Lingjun Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Electrophoresis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xuefei ZhongSchool of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
Ling HaoSchool of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.
Jianfeng LuDepartment of Neuroscience and Department of Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, WI, USA.
Hui YeChina Pharmaceutical University, Nanjing, P. R. China.
Su-Chun ZhangDepartment of Neuroscience and Department of Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, WI, USA.
Lingjun LiSchool of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA.

Funding

Transgenic Human Stem Cell Lines and ReagentsR24NS086604 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI ZHANG, SU-CHUN · 2014 to 2017
$1.1M
NINDS NIH HHS 5R24NS086604NINDS NIH HHS R24 NS086604
6 · The paper itself

Abstract

A CE-ESI-MRM-based assay was developed for targeted analysis of serotonin released by human embryonic stem cells-derived serotonergic neurons in a chemically defined environment. A discontinuous electrolyte system was optimized for pH-mediated online stacking of serotonin. Combining with a liquid-liquid extraction procedure, LOD of serotonin in the Krebs'-Ringer's solution by CE-ESI-MS/MS on a 3D ion trap MS was0.15 ng/mL. The quantitative results confirmed the serotonergic identity of the in vitro developed neurons and the capacity of these neurons to release serotonin in response to stimulus.

Indexed as

Cell LineElectrophoresis, CapillaryHuman Embryonic Stem CellsHumansHydrogen-Ion ConcentrationLiquid-Liquid ExtractionSerotoninSpectrometry, Mass, Electrospray IonizationTime FactorsSerotoninCE-ESI-MRMOnline concentrationSerotonergic neuronSerotonin

Identifiers

PMID26791443
PMCPMC6314833

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.