ArticleMalaria journal2016
UTR introns, antisense RNA and differentially spliced transcripts between Plasmodium yoelii subspecies.
Article in Malaria journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 25 citations in OpenAlex.
- Population genomics and transcriptomics of Plasmodium falciparum in Cambodia and Vietnam uncover key components of the artemisinin resistance genetic background.Nature communications · 2024Article
- Comparative genomics of Plasmodium yoelii nigeriensis N67 and N67C: genome-wide polymorphisms, differential gene expression, and drug resistance.BMC genomics · 2024Article
- Size-dependent enhancement of gene expression by Plasmodium 5'UTR introns.Parasites & vectors · 2024Article
- Immunological characteristics of CD103Parasitology research · 2023Article
- Long-Read Genome Assembly and Gene Model Annotations for the Rodent Malaria ParasitebioRxiv : the preprint server for biology · 2023Article
- The origins, isolation, and biological characterization of rodent malaria parasites.Parasitology international · 2022Review
- Long read single cell RNA sequencing reveals the isoform diversity of Plasmodium vivax transcripts.PLoS neglected tropical diseases · 2022Article
- Genome sequence, transcriptome, and annotation of rodent malaria parasite Plasmodium yoelii nigeriensis N67.BMC genomics · 2021Article
- Dual RNA Sequencing Meta-analysis inmSystems · 2021Article
- Properties and Roles of γδT Cells inFrontiers in cellular and infection microbiology · 2021Article
- A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate.PLoS neglected tropical diseases · 2019Article
- Review
- Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms.Scientific reports · 2017Article
- Increased CD40 Expression Enhances Early STING-Mediated Type I Interferon Response and Host Survival in a Rodent Malaria Model.PLoS pathogens · 2016Article
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe rodent malaria parasite Plasmodium yoelii is an important animal model for studying host-parasite interaction and molecular basis of malaria pathogenesis. Although a draft genome of P. yoelii yoelii YM is available, and RNA sequencing (RNA-seq) data for several rodent malaria species (RMP) were reported recently, variations in coding regions and structure of mRNA transcript are likely present between different parasite strains or subspecies. Sequencing of cDNA libraries from additional parasite strains/subspecies will help improve the gene models and genome annotation.
methodsHere two directional cDNA libraries from mixed blood stages of a subspecies of P. yoelii (P. y. nigeriensis NSM) with or without mefloquine (MQ) treatment were sequenced, and the sequence reads were compared to the genome and cDNA sequences of P. y. yoelii YM in public databases to investigate single nucleotide polymorphisms (SNPs) in coding regions, variations in intron-exon structure and differential splicing between P. yoelii subspecies, and variations in gene expression under MQ pressure.
resultsApproximately 56 million of 100 bp paired-end reads were obtained, providing an average of ~225-fold coverage for the coding regions. Comparison of the sequence reads to the YM genome revealed introns in 5' and 3' untranslated regions (UTRs), altered intron/exon boundaries, alternative splicing, overlapping sense-antisense reads, and potentially new transcripts. Interestingly, comparison of the NSM RNA-seq reads obtained here with those of YM discovered differentially spliced introns; e.g., spliced introns in one subspecies but not the other. Alignment of the NSM cDNA sequences to the YM genome sequence also identified ~84,000 SNPs between the two parasites.
conclusionThe discoveries of UTR introns and differentially spliced introns between P. yoelii subspecies raise interesting questions on the potential role of these introns in regulating gene expression and evolution of malaria parasites.
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