ArticleMicrobial cell factories2016
Mechanism of imidazolium ionic liquids toxicity in Saccharomyces cerevisiae and rational engineering of a tolerant, xylose-fermenting strain.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The effect of DMSO on Saccharomyces cerevisiae yeast with different energy metabolism and antioxidant status.Scientific reports · 2024Article
- A role for ion homeostasis in yeast ionic liquid tolerance.microPublication biology · 2023Article
- Ionic Liquids as Biocompatible Antibacterial Agents: A Case Study on Structure-Related Bioactivity onACS applied bio materials · 2022Article
- Water-soluble saponins accumulate in drought-stressed switchgrass and may inhibit yeast growth during bioethanol production.Biotechnology for biofuels and bioproducts · 2022Article
- Comparative chemical genomic profiling across plant-based hydrolysate toxins reveals widespread antagonism in fitness contributions.FEMS yeast research · 2022Article
- Ionic Liquid-Based Polymer Nanocomposites for Sensors, Energy, Biomedicine, and Environmental Applications: Roadmap to the Future.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Gene Coexpression Connectivity Predicts Gene Targets Underlying High Ionic-Liquid Tolerance in Yarrowia lipolytica.mSystems · 2022Article
- Extrapolation of design strategies for lignocellulosic biomass conversion to the challenge of plastic waste.Journal of industrial microbiology & biotechnology · 2022Article
- Ionic Liquid-Based Materials for Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2021Review
- A high solids field-to-fuel research pipeline to identify interactions between feedstocks and biofuel production.Biotechnology for biofuels · 2021Article
- Process intensification of the ionoSolv pretreatment: effects of biomass loading, particle size and scale-up from 10 mL to 1 L.Scientific reports · 2021Article
- Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system.Scientific reports · 2021Article
- Antibacterial, Antifungal and Ecotoxic Effects of Ammonium and Imidazolium Ionic Liquids Synthesized in Microwaves.Molecules (Basel, Switzerland) · 2020Article
- Improving ionic liquid tolerance in Saccharomyces cerevisiae through heterologous expression and directed evolution of an ILT1 homolog from Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2019Article
- EngineeringBiotechnology for biofuels · 2019Article
- Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC.Microbial cell factories · 2018Article
- The Use of Liquids Ionic Fluids as Pharmaceutically Active Substances Helpful in Combating Nosocomial Infections Induced byInternational journal of molecular sciences · 2018Review
- Natural Variation in the Multidrug Efflux PumpGenetics · 2018Article
- Kluyveromyces marxianus, an Attractive Yeast for Ethanolic Fermentation in the Presence of Imidazolium Ionic Liquids.International journal of molecular sciences · 2018Article
- Chemical genomic guided engineering of gamma-valerolactone tolerant yeast.Microbial cell factories · 2018Article
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Authors and funding
12 authors.
Funding
Abstract
backgroundImidazolium ionic liquids (IILs) underpin promising technologies that generate fermentable sugars from lignocellulose for future biorefineries. However, residual IILs are toxic to fermentative microbes such as Saccharomyces cerevisiae, making IIL-tolerance a key property for strain engineering. To enable rational engineering, we used chemical genomic profiling to understand the effects of IILs on S. cerevisiae.
resultsWe found that IILs likely target mitochondria as their chemical genomic profiles closely resembled that of the mitochondrial membrane disrupting agent valinomycin. Further, several deletions of genes encoding mitochondrial proteins exhibited increased sensitivity to IIL. High-throughput chemical proteomics confirmed effects of IILs on mitochondrial protein levels. IILs induced abnormal mitochondrial morphology, as well as altered polarization of mitochondrial membrane potential similar to valinomycin. Deletion of the putative serine/threonine kinase PTK2 thought to activate the plasma-membrane proton efflux pump Pma1p conferred a significant IIL-fitness advantage. Conversely, overexpression of PMA1 conferred sensitivity to IILs, suggesting that hydrogen ion efflux may be coupled to influx of the toxic imidazolium cation. PTK2 deletion conferred resistance to multiple IILs, including [EMIM]Cl, [BMIM]Cl, and [EMIM]Ac. An engineered, xylose-converting ptk2∆ S. cerevisiae (Y133-IIL) strain consumed glucose and xylose faster and produced more ethanol in the presence of 1 % [BMIM]Cl than the wild-type PTK2 strain. We propose a model of IIL toxicity and resistance.
conclusionsThis work demonstrates the utility of chemical genomics-guided biodesign for development of superior microbial biocatalysts for the ever-changing landscape of fermentation inhibitors.
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