ReviewJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2016
Acetylene hydratase: a non-redox enzyme with tungsten and iron-sulfur centers at the active site.
Review in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Proton-Coupled OAT Triggers C2H2 Activation in a Bioinspired Molybdenum Complex.Journal of the American Chemical Society · 2026Article
- Comparative Study of Aqueous Acid-Base Properties of Tungstocene and Molybdocene Complexes.Organometallics · 2026Article
- Bond Valence Sum Parameters for Analyzing Pyranopterin Tungsten Enzyme Structures.Molecules (Basel, Switzerland) · 2025Article
- L-Cysteine-Catalysed Hydration of Activated Alkynes.Angewandte Chemie (International ed. in English) · 2025Article
- Understanding the Carbyne Formation from CJournal of the American Chemical Society · 2024Article
- Active Site Characterization of aInorganic chemistry · 2024Article
- Vinyl-pyrazole as a biomimetic acetaldehyde surrogate.Chemical communications (Cambridge, England) · 2024Article
- Nucleophiles Target the Tungsten Center Over Acetylene in Biomimetic Models.Inorganic chemistry · 2024Article
- Advancing Our Understanding of Pyranopterin-Dithiolene Contributions to Moco Enzyme Catalysis.Molecules (Basel, Switzerland) · 2023Review
- Dioxygen Activation by a Bioinspired Tungsten(IV) Complex.Inorganic chemistry · 2023Article
- The elements of life: A biocentric tour of the periodic table.Advances in microbial physiology · 2023Article
- Complete Genome Sequence of Rhodococcus opacus Strain MoAcy1 (DSM 44186), an Aerobic Acetylenotroph Isolated from Soil.Microbiology resource announcements · 2022Article
- Synthesis and Reactivity of a Bioinspired Molybdenum(IV) Acetylene Complex.Organometallics · 2021Article
- Acetylene-Fueled Trichloroethene Reductive Dechlorination in a Groundwater Enrichment Culture.mBio · 2021Article
- Got acetylene: a personal research retrospective.FEMS microbes · 2021Article
- Soft Scorpionate Hydridotris(2-mercapto-1-methylimidazolyl) borate) Tungsten-Oxido and -Sulfido Complexes as Acetylene Hydratase Models.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Article
- Activation and Photoinduced Release of Alkynes on a Biomimetic Tungsten Center: The Photochemical Behavior of the W-S-Phoz System.Chemistry (Weinheim an der Bergstrasse, Germany) · 2019Article
- Acetylenotrophy: a hidden but ubiquitous microbial metabolism?FEMS microbiology ecology · 2018Review
- ATP-dependent substrate reduction at an [FeProceedings of the National Academy of Sciences of the United States of America · 2018Article
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1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In living systems, tungsten is exclusively found in microbial enzymes coordinated by the pyranopterin cofactor, with additional metal coordination provided by oxygen and/or sulfur, and/or selenium atoms in diverse arrangements. Prominent examples are formate dehydrogenase, formylmethanofuran dehydrogenase, and aldehyde oxidoreductase all of which catalyze redox reactions. The bacterial enzyme acetylene hydratase (AH) stands out of its class as it catalyzes the conversion of acetylene to acetaldehyde, clearly a non-redox reaction and a reaction distinct from the reduction of acetylene to ethylene by nitrogenase. AH harbors two pyranopterins bound to W, and a [4Fe-4S] cluster. W is coordinated by four dithiolene sulfur atoms, one cysteine sulfur, and one oxygen ligand. AH activity requires a strong reductant suggesting W(IV) as the active oxidation state. Two different types of reaction pathways have been proposed. The 1.26 Å structure reveals a water molecule coordinated to W which could gain a partially positive net charge by the adjacent protonated Asp-13, enabling a direct attack of C2H2. To access the W-Asp site, a substrate channel was evolved distant from where it is found in other members of the DMSOR family. Computational studies of this second shell mechanism led to unrealistically high energy barriers, and alternative pathways were proposed where C2H2 binds directly to W. The architecture of the catalytic cavity, the specificity for C2H2 and the results from site-directed mutagenesis do not support this first shell mechanism. More investigations including structural information on the binding of C2H2 are needed to present a conclusive answer.
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