ArticleNature communications2016
Biochemical characterization of predicted Precambrian RuBisCO.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 144 citations in OpenAlex.
- Enrichment of Hydrogen-Oxidizing Bacteria Using a Hybrid Biological-Inorganic System Under a High COMicroorganisms · 2026Article
- Diversity in Rubisco kinetics and CO2-concentrating mechanisms in Cyanobacteria.Plant physiology · 2026Article
- Functional characterization of bicarbonate transporters from the cyanobacterial SbtA2 family and subsequent expression in tobacco.Journal of experimental botany · 2026Article
- Very high CONAR genomics and bioinformatics · 2026Article
- Heterologous expression in E. coli reveals that bicarbonate transporter BicA2 mediates carbon uptake in marine Prochlorococcus spp.The ISME journal · 2026Article
- Not just Rubisco: integrated strategies to overcome the biochemical limitations of CFrontiers in plant science · 2026Review
- Rubisco is slow across the tree of life.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Inorganic Carbon Acquisition and Photosynthetic Metabolism in Marine Photoautotrophs: A Summary.Plants (Basel, Switzerland) · 2025Review
- Applications of ancestral sequence reconstruction for understanding the evolution of plant specialized metabolism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage.Nature plants · 2024Article
- The genomic potential of photosynthesis in piconanoplankton is functionally redundant but taxonomically structured at a global scale.Science advances · 2024Article
- A systematic exploration of bacterial form I rubisco maximal carboxylation rates.The EMBO journal · 2024Article
- Multisubstrate specificity shaped the complex evolution of the aminotransferase family across the tree of life.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Primordial magnetotaxis in putative giant paleoproterozoic magnetofossils.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Rubisco is evolving for improved catalytic efficiency and COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Deep-branching evolutionary intermediates reveal structural origins of form I rubisco.Current biology : CB · 2023Article
- Insights into the biological control of the carbon isotope signatures of ancient aquatic organisms.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Carbon isotope fractionation by an ancestral rubisco suggests that biological proxies for COProceedings of the National Academy of Sciences of the United States of America · 2023Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The antiquity and global abundance of the enzyme, RuBisCO, attests to the crucial and longstanding role it has played in the biogeochemical cycles of Earth over billions of years. The counterproductive oxygenase activity of RuBisCO has persisted over billions of years of evolution, despite its competition with the carboxylase activity necessary for carbon fixation, yet hypotheses regarding the selective pressures governing RuBisCO evolution have been limited to speculation. Here we report the resurrection and biochemical characterization of ancestral RuBisCOs, dating back to over one billion years ago (Gyr ago). Our findings provide an ancient point of reference revealing divergent evolutionary paths taken by eukaryotic homologues towards improved specificity for CO2, versus the evolutionary emphasis on increased rates of carboxylation observed in bacterial homologues. Consistent with these distinctions, in vivo analysis reveals the propensity of ancestral RuBisCO to be encapsulated into modern-day carboxysomes, bacterial organelles central to the cyanobacterial CO2 concentrating mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.