ArticleBiophysical journal2016
Cis and Trans Cooperativity of E-Cadherin Mediates Adhesion in Biomimetic Lipid Droplets.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Adhesion differentials control the rheology of biomimetic emulsions.Biophysical journal · 2026Article
- Design and optimization of in situ self-functionalizing stress sensors.The European physical journal. E, Soft matter · 2026Article
- Persistent cell contacts enable E-cadherin/HMR-1- and PAR-3-based symmetry breaking within a developing C. elegans epithelium.Developmental cell · 2023Article
- Push-pull mechanics of E-cadherin ectodomains in biomimetic adhesions.Biophysical journal · 2023Article
- A coarse-grained simulation model for colloidal self-assemblySoft matter · 2023Article
- Nucleation of cadherin clusters on cell-cell interfaces.Scientific reports · 2022Article
- Peeling dynamics of fluid membranes bridged by molecular bonds: moving or breaking.Journal of the Royal Society, Interface · 2022Article
- DNA self-organization controls valence in programmable colloid design.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cadherin cis and trans interactions are mutually cooperative.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Ruffles and spikes: Control of tight junction morphology and permeability by claudins.Biochimica et biophysica acta. Biomembranes · 2020Review
- Design and Application of Stimulus-Responsive Droplets and Bubbles Stabilized by Phospholipid Monolayers.Current opinion in colloid & interface science · 2019Article
- Biological tissue-inspired tunable photonic fluid.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cell-cell adhesion interface: orthogonal and parallel forces from contraction, protrusion, and retraction.F1000Research · 2018Review
- Integration of Cadherin Adhesion and Cytoskeleton atCold Spring Harbor perspectives in biology · 2017Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of cell-cell adhesion is important in cell motility, tissue growth, and for the mechanical integrity of tissues. Although the role of active cytoskeleton dynamics in regulating cadherin interactions is crucial in vivo, here we present a biomimetic emulsion system to characterize the passive E-cadherin-mediated adhesion between droplets. The visualization of a three-dimensional assembly of lipid droplets, functionalized with extracellular E-cadherin domains, reveals a hierarchy of homophilic interactions. First, the high interfacial tension of droplets facilitates trans cadherin-cadherin adhesion, which is strong enough to stabilize looser than random close packing configurations. Second, fluorescence enhancement shows that adding clustering agents, such as calcium or chelating ligands, favor the lateral cis adhesion of the already bound cadherin pairs over the clustering of monomer cadherin on the surface. Finally, above a threshold cadherin and calcium concentration, the cis and trans protein interactions become strong enough to trigger and promote droplet fusion. While E-cadherin is not known to participate in cellular fusion, this mechanism is general because replacing calcium with cholesterol to cluster the cadherin-carrying lipids also promotes fusion. These results suggest that passive clustering, via calcium-induced dimerization or membrane ordering, may contribute to the reinforcement of cell-cell contacts. Alternatively, a molecular switch for fusion offers a route to mixing droplet contents and controlling their size in situ.
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