ArticleMolecular plant pathology2017
A quorum sensing-defective mutant of Pectobacterium carotovorum ssp. brasiliense 1692 is attenuated in virulence and unable to occlude xylem tissue of susceptible potato plant stems.
Article in Molecular plant pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 59 citations in OpenAlex.
- A critical review of natural quorum-sensing inhibitors as molecular anti-virulence agents against phytopathogenic bacteria.Archives of microbiology · 2026Review
- Quorum-quenching halotolerant bacteria as biocontrol agents against potato phytopathogens.Scientific reports · 2025Article
- Effects of theBiofilm · 2025Article
- Multimodal Inhibition ofJournal of agricultural and food chemistry · 2025Article
- Gene expression analyses on Dickeya solani strains of diverse virulence levels unveil important pathogenicity factors for this species.Scientific reports · 2025Article
- Article
- Arabidopsis WRKY55 Transcription Factor Enhances Soft Rot Disease Resistance with ORA59.The plant pathology journal · 2024Article
- Impact of Homologous Recombination on Core Genome Evolution and Host Adaptation of Pectobacterium parmentieri.Genome biology and evolution · 2024Article
- Manuka Honey Inhibits Biofilm Formation and Reduces the Expression of the Associated Genes inScientifica · 2024Article
- MzmL, a novel marine derivedFrontiers in microbiology · 2024Article
- The Role of Intercellular Signaling in the Regulation of Bacterial Adaptive Proliferation.International journal of molecular sciences · 2023Article
- Disalicylic Acid Provides Effective Control ofMicroorganisms · 2022Article
- Biofilm Formation and Structure in the Filamentous Fungus Fusarium graminearum, a Plant Pathogen.Microbiology spectrum · 2022Article
- RasI/R Quorum Sensing System Controls the Virulence ofApplied and environmental microbiology · 2022Article
- The Role ofInternational journal of molecular sciences · 2021Article
- Isolation and Characterization of Outer Membrane Vesicles ofMicroorganisms · 2021Article
- The First Polish Isolate of a Novel SpeciesInternational journal of environmental research and public health · 2021Article
- Biosensors Used for Epifluorescence and Confocal Laser Scanning Microscopies to StudyMicroorganisms · 2021Review
- Physiological Basis of Smut Infectivity in the Early Stages of Sugar Cane Colonization.Journal of fungi (Basel, Switzerland) · 2021Review
- Review
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Pectobacterium carotovorum ssp. brasiliense 1692 (Pcb1692) is an important emerging pathogen of potatoes causing blackleg in the field and soft rot during post-harvest storage. Blackleg diseases involve the bacterial colonization of vascular tissue and the formation of aggregates, also known as biofilms. To understand the role of quorum sensing in vascular colonization by Pcb1692, we generated a Pcb1692ΔexpI mutant strain. Inactivation of expI led to the reduced production of plant cell wall-degrading enzymes (PCWDEs), the inability to produce acyl homoserine lactone (AHL) and reduced virulence in potato tubers and stems. Complementation of the mutant strain with the wild-type expI gene in trans successfully restored AHL and PCWDE production as well as virulence. Transmission electron microscopy and in vitro motility assays demonstrated hyperpiliation and loss of flagella and swimming motility in the mutant strain compared with the wild-type Pcb1692. Furthermore, we noted that, in the early stages of infection, Pcb1692 wild-type cells had intact flagella which were shed at the later stages of infection. Confocal laser microscopy of PcbΔexpI-inoculated plants showed that the mutant strain tended to aggregate in intercellular spaces, but was unable to transit to xylem tissue. On the contrary, the wild-type strain was often observed forming aggregates within xylem tissue of potato stems. Gene expression analyses confirmed that flagella are part of the quorum sensing regulon, whereas fimbriae and pili appear to be negatively regulated by quorum sensing. The relative expression levels of other important putative virulence genes, such as those encoding different groups of PCWDEs, were down-regulated in the mutant compared with the wild-type strain.
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