Evidence map›Paper›PMID 26788028›Full record

ArticleMolecular vision2015

Persistence of reduced expression of putative stem cell markers and slow wound healing in cultured diabetic limbal epithelial cells.

Andrei A Kramerov, Mehrnoosh Saghizadeh, Ezra Maguen, Yaron S Rabinowitz, Alexander V Ljubimov

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Molecular vision, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Systemic diseases and the cornea.Experimental eye research · 2021
    Review
  9. Article
  10. Article
  11. Corneal alteration and pathogenesis in diabetes mellitus.International journal of ophthalmology · 2019
    Review
  12. Limbal stem cells: identity, developmental origin, and therapeutic potential.Wiley interdisciplinary reviews. Developmental biology · 2018
    Review
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Andrei A KramerovEye Program, Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA.
Mehrnoosh SaghizadehEye Program, Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA; David Geffen School of Medicine at UCLA, Los Angeles, CA.
Ezra MaguenAmerican Eye Institute, Los Angeles, CA; Jules Stein Eye Institute, UCLA, Los Angeles, CA.
Yaron S RabinowitzEye Program, Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA.
Alexander V LjubimovEye Program, Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA; David Geffen School of Medicine at UCLA, Los Angeles, CA.
Cedars-Sinai Medical Center · USRegenerative Medicine Institute · MXDoheny Eye Institute · US

Funding

Mechanisms of Epithelial Alterations in Diabetic CorneaR01EY013431 · NEI · CEDARS-SINAI MEDICAL CENTER · PI Alexander V Ljubimov · 2001 to 2026
$11.3M
GENETIC FACTORS IN KERATOCONUSR01EY009052 · NEI · CEDARS-SINAI MEDICAL CENTER · PI RABINOWITZ, YARON SOLOMON, ROTTER, JEROME I · 1993 to 2019
$10.9M
Transplantable limbal cells from induced pluripotent stem cellsR01EY023429 · NEI · CEDARS-SINAI MEDICAL CENTER · PI LJUBIMOV, ALEXANDER V · 2013 to 2015
$1.2M
NEI NIH HHS R01 EY009052NEI NIH HHS R01 EY013431NEI NIH HHS R01EY013431NEI NIH HHS R01 EY023429NEI NIH HHS R01EY023429
6 · The paper itself

Abstract

purposeTo examine the expression of putative limbal epithelial stem cell (LESC) markers and wound healing rates in primary healthy and diabetic human limbal epithelial cells (LECs) cultured on different substrata.

methodsPrimary limbal epithelial cells were isolated from human autopsy corneas and discarded corneoscleral rims with dispase II treatment. LECs were cultured in EpiLife medium on human amniotic membrane (AM) denuded with mild alkali treatment, on plastic dishes and on glass slides coated with a mixture of human fibronectin, collagen type IV, and laminin (FCL). Cultured LECs were fixed in p-formaldehyde or methanol, and the expression of the putative LESC markers ΔNp63α, PAX6, and ABCG2 and keratins K12, K15, and K17 was examined with immunostaining. Wound healing was evaluated in scratch wound assay in LECs cultured on FCL-coated plates 20 h after wounding.

resultsLECs cultured on denuded AM expressed ΔNp63α, PAX6 (both showed nuclear staining), K15, K17 (cytoskeleton staining), and ABCG2 (cytoplasmic and/or plasma membrane staining). LECs cultured on FCL-coated slides also expressed these markers, whereas no expression was detected for differentiated corneal epithelial cell marker K12. Decreased expression of LESC markers was observed in diabetic LECs compared to healthy LECs cultured on the FCL-coated slides. This reduction was most prominent for K15 and K17. Diabetic LECs were found to heal scratch wounds slower than healthy cells in accordance with previous results in corneal organ cultures.

conclusionsHealthy human LECs cultured either on AM or FCL-coated slides preserved LESC marker expression. The observed reduction in LESC marker expression and slower wound healing in cultured diabetic LECs are in line with our earlier reports and may account for diabetic LESC dysfunction and clinically observed impaired corneal epithelial wound healing.

Indexed as

AgedAged, 80 and overBiomarkersCells, CulturedCorneal DiseasesCulture MediaDiabetes ComplicationsEpithelial CellsEpithelium, CornealFemaleHumansLimbus CorneaeMaleMiddle AgedStem CellsWound HealingBiomarkersCulture Media

Identifiers

PMID26788028
PMCPMC4704770
OpenAlexW2285582823

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.