ArticleProceedings of the National Academy of Sciences of the United States of America2016
Mobile small RNAs regulate genome-wide DNA methylation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
102 citing papers in PubMed, 231 citations in OpenAlex.
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- Cell-type-specific ablation of siRNAs by Arabidopsis RTL1 reveals a role of phloem companion cells in systemic post-transcriptional gene silencing.Nucleic acids research · 2026Article
- Small RNAs as systemic signals in plant defense: mechanisms, challenges, and future directions.Molecular biology reports · 2026Review
- Small RNAs regulation and genomic harmony: insights into allopolyploid evolution in marsh orchids (Dactylorhiza).The New phytologist · 2026Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Long-distance transport of siRNAs with functional roles in pollen development.Nature plants · 2026Article
- Three decades of mobile RNA silencing within plants: what have we learnt?Journal of experimental botany · 2026Review
- Mobile small RNAs as mediators of stress-induced epigenomic remodeling and systemic stress adaptation.Frontiers in epigenetics and epigenomics · 2026Review
- DNA methylation contributes to plant acclimation to naturally fluctuating light.The New phytologist · 2025Article
- Epigenetic crop improvement: Integrating ENCODE strategies into horticultural breeding.Horticulture research · 2025Article
- Epigenetic regulation modulates seasonal temperature-dependent growth of soybean in southern China.Plant biotechnology journal · 2025Article
- Rootstock of distant hybrid activates transcription factors of scion cultivar to promote growth and yield in walnut.Plant biotechnology journal · 2025Article
- RNA interference shapes stress responses inFrontiers in plant science · 2025Review
- Classification of and detection techniques for RNAi-induced effects in GM plants.Frontiers in plant science · 2025Review
- Deep Sequencing Analysis of Virome Components, Viral Gene Expression and Antiviral RNAi Responses inInternational journal of molecular sciences · 2024Article
- Article
- Heritable changes of epialleles near genes in maize can be triggered in the absence of CHH methylation.Plant physiology · 2024Article
- The plant siRNA landscape.The Plant cell · 2024Review
- Global hypermethylation of the N6-methyladenosine RNA modification associated with apple heterografting.Plant physiology · 2023Article
- The mRNA mobileome: challenges and opportunities for deciphering signals from the noise.The Plant cell · 2023Review
42 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
RNA silencing at the transcriptional and posttranscriptional levels regulates endogenous gene expression, controls invading transposable elements (TEs), and protects the cell against viruses. Key components of the mechanism are small RNAs (sRNAs) of 21-24 nt that guide the silencing machinery to their nucleic acid targets in a nucleotide sequence-specific manner. Transcriptional gene silencing is associated with 24-nt sRNAs and RNA-directed DNA methylation (RdDM) at cytosine residues in three DNA sequence contexts (CG, CHG, and CHH). We previously demonstrated that 24-nt sRNAs are mobile from shoot to root in Arabidopsis thaliana and confirmed that they mediate DNA methylation at three sites in recipient cells. In this study, we extend this finding by demonstrating that RdDM of thousands of loci in root tissues is dependent upon mobile sRNAs from the shoot and that mobile sRNA-dependent DNA methylation occurs predominantly in non-CG contexts. Mobile sRNA-dependent non-CG methylation is largely dependent on the DOMAINS REARRANGED METHYLTRANSFERASES 1/2 (DRM1/DRM2) RdDM pathway but is independent of the CHROMOMETHYLASE (CMT)2/3 DNA methyltransferases. Specific superfamilies of TEs, including those typically found in gene-rich euchromatic regions, lose DNA methylation in a mutant lacking 22- to 24-nt sRNAs (dicer-like 2, 3, 4 triple mutant). Transcriptome analyses identified a small number of genes whose expression in roots is associated with mobile sRNAs and connected to DNA methylation directly or indirectly. Finally, we demonstrate that sRNAs from shoots of one accession move across a graft union and target DNA methylation de novo at normally unmethylated sites in the genomes of root cells from a different accession.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.