ArticleProceedings of the National Academy of Sciences of the United States of America2016
Intrinsic regulation of FIC-domain AMP-transferases by oligomerization and automodification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Inhibition of FicD-mediated AMPylation and deAMPylation by isoprenoid diphosphates.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- AMPylation: key roles in biological regulation across prokaryotes and eukaryotes.Frontiers in cellular and infection microbiology · 2026Review
- Activity-based Tools for Interrogating Host Biology During Infection.Israel journal of chemistry · 2023Article
- A secreted effector with a dual role as a toxin and as a transcriptional factor.Nature communications · 2022Article
- Modelling evolutionary pathways for commensalism and hypervirulence inMicrobial genomics · 2021Article
- Evolutionary Diversification of Host-TargetedMicroorganisms · 2021Article
- Prokaryote toxin-antitoxin modules: Complex regulation of an unclear function.Protein science : a publication of the Protein Society · 2021Review
- Fic and non-Fic AMPylases: protein AMPylation in metazoans.Open biology · 2021Article
- Fic Proteins Inhibit the Activity of Topoisomerase IV by AMPylation in Diverse Bacteria.Frontiers in microbiology · 2020Article
- An oligomeric state-dependent switch in the ER enzyme FICD regulates AMPylation and deAMPylation of BiP.The EMBO journal · 2019Article
- CryoAPEX - an electron tomography tool for subcellular localization of membrane proteins.Journal of cell science · 2019Article
- A CaNature communications · 2019Article
- A high-throughput integrated microfluidics method enables tyrosine autophosphorylation discovery.Communications biology · 2019Article
- Enzymes Involved in AMPylation and deAMPylation.Chemical reviews · 2018Review
- rAMPing Up Stress Signaling: Protein AMPylation in Metazoans.Trends in cell biology · 2017Review
- Fic Proteins ofFrontiers in microbiology · 2017Article
- A Novel Fic (Filamentation Induced by cAMP) Protein from Clostridium difficile Reveals an Inhibitory Motif-independent Adenylylation/AMPylation Mechanism.The Journal of biological chemistry · 2016Article
- The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones.PLoS genetics · 2016Article
- Article
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filamentation induced by cyclic AMP (FIC)-domain enzymes catalyze adenylylation or other posttranslational modifications of target proteins to control their function. Recently, we have shown that Fic enzymes are autoinhibited by an α-helix (αinh) that partly obstructs the active site. For the single-domain class III Fic proteins, the αinh is located at the C terminus and its deletion relieves autoinhibition. However, it has remained unclear how activation occurs naturally. Here, we show by structural, biophysical, and enzymatic analyses combined with in vivo data that the class III Fic protein NmFic from Neisseria meningitidis gets autoadenylylated in cis, thereby autonomously relieving autoinhibition and thus allowing subsequent adenylylation of its target, the DNA gyrase subunit GyrB. Furthermore, we show that NmFic activation is antagonized by tetramerization. The combination of autoadenylylation and tetramerization results in nonmonotonic concentration dependence of NmFic activity and a pronounced lag phase in the progress of target adenylylation. Bioinformatic analyses indicate that this elaborate dual-control mechanism is conserved throughout class III Fic proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.