ArticleInfection and immunity2016
The fruRBA Operon Is Necessary for Group A Streptococcal Growth in Fructose and for Resistance to Neutrophil Killing during Growth in Whole Human Blood.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
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- Determinants of bacterial survival and proliferation in blood.FEMS microbiology reviews · 2024Review
- luxS contributes to intramacrophage survival of Streptococcus agalactiae by positively affecting the expression of fruRKI operon.Veterinary research · 2023Article
- Contribution of Streptococcus pyogenes M87 protein to innate immune resistance and virulence.Microbial pathogenesis · 2022Article
- BifA Triggers Phosphorylation of Ezrin to BenefitBiomedicines · 2022Article
- Role of FruR transcriptional regulator in virulence of Listeria monocytogenes and identification of its regulon.PloS one · 2022Article
- Streptococcus pyogenes upregulates arginine catabolism to exert its pathogenesis on the skin surface.Cell reports · 2021Article
- Subpopulation behaviors in lactose metabolism by Streptococcus mutans.Molecular microbiology · 2021Article
- Phosphotransferase System Uptake and Metabolism of the β-Glucoside Salicin Impact Group A Streptococcal Bloodstream Survival and Soft Tissue Infection.Infection and immunity · 2020Article
- Carbon source regulates polysaccharide capsule biosynthesis inThe Journal of biological chemistry · 2019Article
- Integrated analysis of population genomics, transcriptomics and virulence provides novel insights into Streptococcus pyogenes pathogenesis.Nature genetics · 2019Article
- Biology of Oral Streptococci.Microbiology spectrum · 2018Review
- The Fructose-Specific Phosphotransferase System of Klebsiella pneumoniae Is Regulated by Global Regulator CRP and Linked to Virulence and Growth.Infection and immunity · 2018Article
- Glucose Levels Alter the Mga Virulence Regulon in the Group A Streptococcus.Scientific reports · 2018Article
- Route of Glucose Uptake in the Group a Streptococcus Impacts SLS-Mediated Hemolysis and Survival in Human Blood.Frontiers in cellular and infection microbiology · 2018Article
- Coordinated Regulation of the EIIApplied and environmental microbiology · 2017Article
- Genome-wide discovery of novel M1T1 group A streptococcal determinants important for fitness and virulence during soft-tissue infection.PLoS pathogens · 2017Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial pathogens rely on the availability of nutrients for survival in the host environment. The phosphoenolpyruvate-phosphotransferase system (PTS) is a global regulatory network connecting sugar uptake with signal transduction. Since the fructose PTS has been shown to impact virulence in several streptococci, including the human pathogen Streptococcus pyogenes(the group A Streptococcus[GAS]), we characterized its role in carbon metabolism and pathogenesis in the M1T1 strain 5448. Growth in fructose as a sole carbon source resulted in 103 genes affected transcriptionally, where the frulocus (fruRBA) was the most induced. Reverse transcriptase PCR showed that fruRBA formed an operon which was repressed by FruR in the absence of fructose, in addition to being under carbon catabolic repression. Growth assays and carbon utilization profiles revealed that although the entire fruoperon was required for growth in fructose, FruA was the main transporter for fructose and also was involved in the utilization of three additional PTS sugars: cellobiose, mannitol, and N-acetyl-D-galactosamine. The inactivation of sloR, a fruA homolog that also was upregulated in the presence of fructose, failed to reveal a role as a secondary fructose transporter. Whereas the ability of both ΔfruR and ΔfruB mutants to survive in the presence of whole human blood or neutrophils was impaired, the phenotype was not reproduced in murine whole blood, and those mutants were not attenuated in a mouse intraperitoneal infection. Since the ΔfruA mutant exhibited no phenotype in the human or mouse assays, we propose that FruR and FruB are important for GAS survival in a human-specific environment.
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