ArticleInfection and immunity2016
Genes Required for the Fitness of Salmonella enterica Serovar Typhimurium during Infection of Immunodeficient gp91-/- phox Mice.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Serine Acetyltransferase fromInternational journal of molecular sciences · 2026Article
- Multivalent vaccines for invasiveInfection and immunity · 2026Review
- High-throughput fitness experiments reveal specific vulnerabilities of human-adapted Salmonella during stress and infection.Nature genetics · 2024Article
- Article
- Antibodies and Protection in SystemicInfection and immunity · 2020Review
- Identification of genes required for the fitness ofMicrobial genomics · 2020Article
- Metabolic and genetic basis for auxotrophies in Gram-negative species.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Identification of Drug Resistance Determinants in a Clinical Isolate of Pseudomonas aeruginosa by High-Density Transposon Mutagenesis.Antimicrobial agents and chemotherapy · 2020Article
- BacFITBase: a database to assess the relevance of bacterial genes during host infection.Nucleic acids research · 2020Article
- Retrospective application of transposon-directed insertion-site sequencing to investigate niche-specific virulence of Salmonella Typhimurium in cattle.BMC genomics · 2019Article
- The Essential Genome ofmBio · 2018Article
- Within-host spatiotemporal dynamics of systemic Salmonella infection during and after antimicrobial treatment.The Journal of antimicrobial chemotherapy · 2017Article
- Defining the ABC of gene essentiality in streptococci.BMC genomics · 2017Article
- The secondary resistome of multidrug-resistant Klebsiella pneumoniae.Scientific reports · 2017Article
- Article
- Animal Models for Salmonellosis: Applications in Vaccine Research.Clinical and vaccine immunology : CVI · 2016Review
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Salmonella enterica causes systemic diseases (typhoid and paratyphoid fever), nontyphoidal septicemia (NTS), and gastroenteritis in humans and other animals worldwide. An important but underrecognized emerging infectious disease problem in sub-Saharan Africa is NTS in children and immunocompromised adults. A current goal is to identify Salmonella mutants that are not pathogenic in the absence of key components of the immune system such as might be found in immunocompromised hosts. Such attenuated strains have the potential to be used as live vaccines. We have used transposon-directed insertion site sequencing (TraDIS) to screen mutants of Salmonella enterica serovar Typhimurium for their ability to infect and grow in the tissues of wild-type and immunodeficient mice. This was to identify bacterial genes that might be deleted for the development of live attenuated vaccines that would be safer to use in situations and/or geographical areas where immunodeficiencies are prevalent. The relative fitness of each of 9,356 transposon mutants, representing mutations in 3,139 different genes, was determined in gp91(-/-) phox mice. Mutations in certain genes led to reduced fitness in both wild-type and mutant mice. To validate these results, these genes were mutated by allelic replacement, and resultant mutants were retested for fitness in the mice. A defined deletion mutant of cysE was attenuated in C57BL/6 wild-type mice and immunodeficient gp91(-/-) phox mice and was effective as a live vaccine in wild-type mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.