ArticleTree physiology2016
Moderate stress responses and specific changes in polyamine metabolism characterize Scots pine somatic embryogenesis.
Article in Tree physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 45 citations in OpenAlex.
- Thiourea and arginine synergistically preserve redox homeostasis and ionic balance for alleviating salinity stress in wheat.Scientific reports · 2024Article
- Physiological, Metabolic, and Transcriptomic Analyses Reveal Mechanisms of Proliferation and Somatic Embryogenesis of Litchi (International journal of molecular sciences · 2024Article
- HInternational journal of molecular sciences · 2024Article
- Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis.Plants (Basel, Switzerland) · 2022Article
- The humidity level matters during the desiccation of Norway spruce somatic embryos.Frontiers in plant science · 2022Article
- Changing winter climate and snow conditions induce various transcriptional stress responses in Scots pine seedlings.Frontiers in plant science · 2022Article
- Proteomic Analysis ofFrontiers in plant science · 2022Article
- Leaf transcriptomic signatures for somatic embryogenesis potential of Elaeis guineensis.Plant cell reports · 2021Article
- Article
- Conjugated Polyamines in Root Plasma Membrane Enhanced the Tolerance of Plum Seedling to Osmotic Stress by Stabilizing Membrane Structure and Therefore Elevating HFrontiers in plant science · 2021Article
- The Chemical Environment at Maturation Stage inFrontiers in plant science · 2021Article
- Biologia futura: the role of polyamine in plant science.Biologia futura · 2020Review
- Article
- Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton.International journal of molecular sciences · 2019Article
- Signaling Overview of Plant Somatic Embryogenesis.Frontiers in plant science · 2019Review
- Thermospermine Synthase (Frontiers in plant science · 2019Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Somatic embryogenesis (SE) is one of the methods with the highest potential for the vegetative propagation of commercially important coniferous species. However, many conifers, including Scots pine (Pinus sylvestris L.), are recalcitrant to SE and a better understanding of the mechanisms behind the SE process is needed. In Scots pine SE cultures, embryo production is commonly induced by the removal of auxin, addition of abscisic acid (ABA) and the desiccation of cell masses by polyethylene glycol (PEG). In the present study, we focus on the possible link between the induction of somatic embryo formation and cellular stress responses such as hydrogen peroxide protection, DNA repair, changes in polyamine (PA) metabolism and autophagy. Cellular PA contents and the expression of the PA metabolism genes arginine decarboxylase (ADC), spermidine synthase (SPDS), thermospermine synthase (ACL5) and diamine oxidase (DAO) were analyzed, as well as the expression of catalase (CAT), DNA repair genes (RAD51, KU80) and autophagy-related genes (ATG5, ATG8) throughout the induction of somatic embryo formation in Scots pine SE cultures. Among the embryo-producing SE lines, the expression of ADC, SPDS, ACL5, DAO, CAT, RAD51, KU80 and ATG8 showed consistent profiles. Furthermore, the overall low expression of the stress-related genes suggests that cells in those SE lines were not stressed but recognized the ABA+PEG treatment as a signal to trigger the embryogenic pathway. In those SE lines that were unable to produce embryos, cells seemed to experience the ABA+PEG treatment mostly as osmotic stress and activated a wide range of stress defense mechanisms. Altogether, our results suggest that the direction to the embryogenic pathway is connected with cellular stress responses in Scots pine SE cultures. Thus, the manipulation of stress response pathways may provide a way to enhance somatic embryo production in recalcitrant Scots pine SE lines.
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