ArticleEpigenetics2016
Comparison of Methyl-capture Sequencing vs. Infinium 450K methylation array for methylome analysis in clinical samples.
Article in Epigenetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The association between prenatal famine, DNA methylation and mental disorders: a systematic review and meta-analysis.Clinical epigenetics · 2023Pooled it
- Multi-Ancestry Epigenome-Wide Meta-Analysis Identifies Novel Bulk and Cell-Type-Specific Epigenetic Markers of Asthma with Severe Exacerbations.medRxiv : the preprint server for health sciences · 2026Article
- Genome-wide extraction of differentially methylated DNA regions using adapter-anchored proximity primers.Nucleic acids research · 2026Article
- DNA methylation as a biomarker of prenatal exposures: current challenges and opportunities.Environmental epigenetics · 2026Review
- Validation of target-enriched enzymatic methylation sequencing for brain tumor classification from formalin-fixed paraffin embedded-derived DNA.Brain pathology (Zurich, Switzerland) · 2025Article
- Pre-existing DNA methylation signatures in the prefrontal cortex of alcohol-naïve nonhuman primates define neural vulnerability for future risky ethanol consumption.Neurobiology of disease · 2025Article
- Genome-wide DNA methylation analysis related to ALS patient progression and survival.Journal of neurology · 2024Article
- Computational deconvolution of DNA methylation data from mixed DNA samples.Briefings in bioinformatics · 2024Review
- Accurate prediction of pan-cancer types using machine learning with minimal number of DNA methylation sites.Journal of molecular cell biology · 2023Article
- Epigenome-Wide Association Studies of the Fractional Exhaled Nitric Oxide and Bronchodilator Drug Response in Moderate-to-Severe Pediatric Asthma.Biomedicines · 2023Article
- Comparison of DNA methylation measurements from EPIC BeadChip and SeqCap targeted bisulphite sequencing in PON1 and nine additional candidate genes.Epigenetics · 2022Article
- Human non-CpG methylation patterns display both tissue-specific and inter-individual differences suggestive of underlying function.Epigenetics · 2022Article
- ImmuMethy, a database of DNA methylation plasticity at a single cytosine resolution in human blood and immune cells.Database : the journal of biological databases and curation · 2022Article
- Toxicoepigenetics for Risk Assessment: Bridging the Gap Between Basic and Regulatory Science.Epigenetics insights · 2022Article
- Brush swab as a noninvasive surrogate for tissue biopsies in epigenomic profiling of oral cancer.Biomarker research · 2021Article
- Repeatability of methylation measures using a QIAseq targeted methyl panel and comparison with the Illumina HumanMethylation450 assay.BMC research notes · 2021Article
- Genetic and in utero environmental contributions to DNA methylation variation in placenta.Human molecular genetics · 2021Article
- Detecting Differentially Methylated Promoters in Genes Related to Disease Phenotypes Using R.Bio-protocol · 2021Article
- Comparison of methylation capture sequencing and Infinium MethylationEPIC array in peripheral blood mononuclear cells.Epigenetics & chromatin · 2020Article
- Systemic Investigation of Promoter-wide Methylome and Genome Variations in Gout.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interindividual variability in the epigenome has gained tremendous attention for its potential in pathophysiological investigation, disease diagnosis, and evaluation of clinical intervention. DNA methylation is the most studied epigenetic mark in epigenome-wide association studies (EWAS) as it can be detected from limited starting material. Infinium 450K methylation array is the most popular platform for high-throughput profiling of this mark in clinical samples, as it is cost-effective and requires small amounts of DNA. However, this method suffers from low genome coverage and errors introduced by probe cross-hybridization. Whole-genome bisulfite sequencing can overcome these limitations but elevates the costs tremendously. Methyl-Capture Sequencing (MC Seq) is an attractive intermediate solution to increase the methylome coverage in large sample sets. Here we first demonstrate that MC Seq can be employed using DNA amounts comparable to the amounts used for Infinium 450K. Second, to provide guidance when choosing between the 2 platforms for EWAS, we evaluate and compare MC Seq and Infinium 450K in terms of coverage, technical variation, and concordance of methylation calls in clinical samples. Last, since the focus in EWAS is to study interindividual variation, we demonstrate the utility of MC Seq in studying interindividual variation in subjects from different ethnicities.
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