ArticlePlant molecular biology2016
Cyclic AMP deficiency negatively affects cell growth and enhances stress-related responses in tobacco Bright Yellow-2 cells.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- "Cyclic nucleotides in plants: from obscure messengers to central regulators".Frontiers in plant science · 2025Review
- The cAMP-dependent phosphorylation footprint in response to heat stress.Plant cell reports · 2024Article
- The Effect of Bacterial AHL on the Cyclic Adenosine Monophosphate Content in Plants According to High-Performance Liquid Chromatography.Molecules (Basel, Switzerland) · 2024Article
- Plant adenylate cyclases have come full circle.Nature plants · 2023Review
- A triphosphate tunnel metalloenzyme from pear (PbrTTM1) moonlights as an adenylate cyclase.Frontiers in plant science · 2023Article
- The simultaneous perception of self- and non-self-danger signals potentiates plant innate immunity responses.Planta · 2022Article
- Molecular Targets and Biological Functions of cAMP Signaling inBiomolecules · 2021Article
- Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Cold Tolerance in Rapeseed (Frontiers in plant science · 2021Article
- Functional Crypto-Adenylate Cyclases Operate in Complex Plant Proteins.Frontiers in plant science · 2021Article
- Cyclic AMP: A Polyhedral Signalling Molecule in Plants.International journal of molecular sciences · 2020Review
- Interrogating cyclic AMP signaling using optical approaches.Cell calcium · 2017Review
- The Mammalian Peptide Adrenomedullin Acts as a Growth Factor in Tobacco Plants.Frontiers in physiology · 2017Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyclic adenosine 3',5'-monophosphate (cAMP) is a recognized second messenger; however, knowledge of cAMP involvement in plant physiological processes originates primarily from pharmacological studies. To obtain direct evidence for cAMP function in plants, tobacco Bright Yellow-2 (BY-2) cells were transformed with the cAMP sponge, which is a genetically encoded tool that reduces cAMP availability. BY-2 cells expressing the cAMP sponge (cAS cells), showed low levels of free cAMP and exhibited growth inhibition that was not proportional to the cAMP sponge transcript level. Growth inhibition in cAS cells was closely related to the precocious inhibition of mitosis due to a delay in cell cycle progression. The cAMP deficiency also enhanced antioxidant systems. Remarkable changes occurred in the cAS proteomic profile compared with that of wild-type (WT) cells. Proteins involved in translation, cytoskeletal organization, and cell proliferation were down-regulated, whereas stress-related proteins were up-regulated in cAS cells. These results support the hypothesis that BY-2 cells sense cAMP deficiency as a stress condition. Finally, many proteasome subunits were differentially expressed in cAS cells compared with WT cells, indicating that cAMP signaling broadly affects protein degradation via the ubiquitin/proteasome pathway.
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Registered trials
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