ArticleThe Journal of biological chemistry2016
Type 3 Secretion Translocators Spontaneously Assemble a Hexadecameric Transmembrane Complex.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Mechanistic blockade ofAntimicrobial agents and chemotherapy · 2025Article
- Novel humanized anti-PcrV monoclonal antibody COT-143 protects mice from lethalAntimicrobial agents and chemotherapy · 2024Article
- The sequence of events of enteropathogeniciScience · 2024Article
- Macrophage innate immune responses delineate between defective translocon assemblies produced byVirulence · 2023Article
- Characterization of translocon proteins in the type III secretion system of Lawsonia intracellularis.Veterinary research · 2023Article
- Virulence and Metabolism Crosstalk: Impaired Activity of the Type Three Secretion System (T3SS) in aInternational journal of molecular sciences · 2023Article
- Quercetin Attenuates the Production of Pro-Inflammatory Cytokines in H292 Human Lung Epithelial Cells Infected withJournal of microbiology and biotechnology · 2023Article
- Recent insights into type-3 secretion system injectisome structure and mechanism of human enteric pathogens.Current opinion in microbiology · 2023Review
- Transmembrane substrates of type three secretion system injectisomes.Microbiology (Reading, England) · 2023Review
- Article
- Evolutionary Conservation, Variability, and Adaptation of Type III Secretion Systems.The Journal of membrane biology · 2022Review
- Transmembrane domains of type III-secreted proteins affect bacterial-host interactions in enteropathogenicVirulence · 2021Article
- The type 3 secretion system requires actin polymerization to open translocon pores.PLoS pathogens · 2021Article
- Topological analysis of type 3 secretion translocons in native membranes.Methods in enzymology · 2021Article
- The type III secretion system needle, tip, and translocon.Protein science : a publication of the Protein Society · 2019Review
- On the road to structure-based development of anti-virulence therapeutics targeting the type III secretion system injectisome.MedChemComm · 2019Review
- Visualization of translocons in Yersinia type III protein secretion machines during host cell infection.PLoS pathogens · 2018Article
- Visualization of the type III secretion mediatedeLife · 2018Article
- TheThe Journal of biological chemistry · 2018Article
- Pore-forming activity of the Pseudomonas aeruginosa type III secretion system translocon alters the host epigenome.Nature microbiology · 2018Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A type 3 secretion system is used by many bacterial pathogens to inject proteins into eukaryotic cells. Pathogens insert a translocon complex into the target eukaryotic membrane by secreting two proteins known as translocators. How these translocators form a translocon in the lipid bilayer and why both proteins are required remains elusive. Pseudomonas aeruginosa translocators PopB and PopD insert pores into membranes forming homo- or hetero-complexes of undetermined stoichiometry. Single-molecule fluorescence photobleaching experiments revealed that PopD formed mostly hexameric structures in membranes, whereas PopB displayed a bi-modal distribution with 6 and 12 subunits peaks. However, individually the proteins are not functional for effector translocation. We have found that when added together, the translocators formed distinct hetero-complexes containing 8 PopB and 8 PopD molecules. Thus, the interaction between PopB and PopD guide the assembly of a unique hetero-oligomer in membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.