Evidence map›Paper›PMID 26785911›Full record

ArticleStem cells and development2016

Smooth Muscle Precursor Cells Derived from Human Pluripotent Stem Cells for Treatment of Stress Urinary Incontinence.

Zhe Wang, Yan Wen, Yan Hui Li, Yi Wei, Morgaine Green, Prachi Wani, Pengbo Zhang, Renee Reijo Pera, Bertha Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
6.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 53 citations in OpenAlex.

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  5. Prospects for use of bioengineered tissue from stem cells in gynecology.Acta obstetricia et gynecologica Scandinavica · 2023
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  20. Bioengineering Approaches for Bladder Regeneration.International journal of molecular sciences · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Zhe Wang1 Department of OB/GYN, Stanford University School of Medicine , Stanford, California.
Yan Wen1 Department of OB/GYN, Stanford University School of Medicine , Stanford, California.
Yan Hui Li1 Department of OB/GYN, Stanford University School of Medicine , Stanford, California.
Yi Wei1 Department of OB/GYN, Stanford University School of Medicine , Stanford, California.
Morgaine Green1 Department of OB/GYN, Stanford University School of Medicine , Stanford, California.
Prachi Wani1 Department of OB/GYN, Stanford University School of Medicine , Stanford, California.
Pengbo Zhang4 Department of Pathology, Stanford University School of Medicine , Stanford, California.
Renee Reijo Pera5 Department of Cell Biology and Neurosciences and Department of Chemistry and Biochemistry, Montana State University , Bozeman, Montana.
Bertha Chen1 Department of OB/GYN, Stanford University School of Medicine , Stanford, California.
Stanford University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is great interest in using stem cells (SC) to regenerate a deficient urethral sphincter in patients with urinary incontinence. The smooth muscle component of the sphincter is a significant contributor to sphincter function. However, current translational efforts for sphincter muscle restoration focus only on skeletal muscle regeneration because they rely on adult mesenchymal SC as cell source. These adult SC do not yield sufficient smooth muscle cells (SMCs) for transplantation. We may be able to overcome this limitation by using pluripotent stem cell (PSC) to derive SMCs. Hence, we sought to investigate whether smooth muscle precursor cells (pSMCs) derived from human PSCs can restore urethral function in an animal model generated by surgical urethrolysis and ovariectomy. Rats were divided into four groups: control (no intervention), sham saline (surgery + saline injection), bladder SMC (surgery + human bladder SMC injection), and treatment (surgery + pSMC injection, which includes human embryonic stem cell (hESC) H9-derived pSMC, episomal reprogrammed induced pluripotent stem cells (iPSCs)-derived pSMC, or viral reprogrammed iPSC-derived pSMC). pSMCs (2 × 10(6) cells/rat) were injected periurethrally 3 weeks postsurgery. Leak point pressure (LPP) and baseline external urethral sphincter electromyography were measured 5 weeks postinjection. Both iPSC-derived pSMC treatment groups showed significantly higher LPP compared to the sham saline group, consistent with restoration of urethral sphincter function. While the difference between the H9-derived pSMC treatment and sham saline group was not significant, it did show a trend toward restoration of the LPP to the level of intact controls. Our data indicate that pSMCs derived from human PSCs (hESC and iPSC) can restore sphincter function.

Indexed as

Stem Cell TransplantationAnimalsCells, CulturedFemaleHumansMyoblastsMyocytes, Smooth MusclePluripotent Stem CellsRatsUrinary Incontinence, Stress

Identifiers

PMID26785911
PMCPMC4818543
OpenAlexW2398063748

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.