ArticleScientific reports2016
Biophysical regulation of Chlamydia pneumoniae-infected monocyte recruitment to atherosclerotic foci.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Cholesterol depletion decreases adhesion of non-small cell lung cancer cells to E-selectin.American journal of physiology. Cell physiology · 2023Article
- Interplay of receptor-ligand binding and lipid domain formation during cell adhesion.Frontiers in molecular biosciences · 2022Review
- Nicotine Affects Murine Aortic Stiffness and Fatigue Response During Supraphysiological Cycling.Journal of biomechanical engineering · 2022Article
- Multiparametric biophysical profiling of red blood cells in malaria infection.Communications biology · 2021Article
- Interplay Between Receptor-Ligand Binding and Lipid Domain Formation Depends on the Mobility of Ligands in Cell-Substrate Adhesion.Frontiers in molecular biosciences · 2021Article
- Delicate Role of PD-L1/PD-1 Axis in Blood Vessel Inflammatory Diseases: Current Insight and Future Significance.International journal of molecular sciences · 2020Review
- Chronic Inflammatory Diseases at Secondary Sites Ensuing Urogenital or PulmonaryMicroorganisms · 2020Review
- Influence of membrane cholesterol on monocyte chemotaxis.Cellular immunology · 2018Article
- Cholesterol Regulates Monocyte Rolling through CD44 Distribution.Biophysical journal · 2017Article
- Manipulation of Host Cholesterol by Obligate Intracellular Bacteria.Frontiers in cellular and infection microbiology · 2017Review
- Imaging flow cytometry analysis of intracellular pathogens.Methods (San Diego, Calif.) · 2017Review
- CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima.Nature immunology · 2016Article
- Biophysical and Biochemical Outcomes of Chlamydia pneumoniae Infection Promotes Pro-atherogenic Matrix Microenvironment.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chlamydia pneumoniae infection is implicated in atherosclerosis although the contributory mechanisms are poorly understood. We hypothesize that C. pneumoniae infection favors the recruitment of monocytes to atherosclerotic foci by altering monocyte biophysics. Primary, fresh human monocytes were infected with C. pneumoniae for 8 h, and the interactions between monocytes and E-selectin or aortic endothelium under flow were characterized by video microscopy and image analysis. The distribution of membrane lipid rafts and adhesion receptors were analyzed by imaging flow cytometry. Infected cells rolled on E-selectin and endothelial surfaces, and this rolling was slower, steady and uniform compared to uninfected cells. Infection decreases cholesterol levels, increases membrane fluidity, disrupts lipid rafts, and redistributes CD44, which is the primary mediator of rolling interactions. Together, these changes translate to higher firm adhesion of infected monocytes on endothelium, which is enhanced in the presence of LDL. Uninfected monocytes treated with LDL or left untreated were used as baseline control. Our results demonstrate that the membrane biophysical changes due to infection and hyperlipidemia are one of the key mechanisms by which C. pneumoniae can exacerbate atherosclerotic pathology. These findings provide a framework to characterize the role of 'infectious burden' in the development and progression of atherosclerosis.
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Registered trials
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