ArticleThe ISME journal2016
Modulation of host ROS metabolism is essential for viral infection of a bloom-forming coccolithophore in the ocean.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 97 citations in OpenAlex.
- The biogeography of giant viruses and interactions with Eukaryotes across European lakes.Nature communications · 2026Article
- Algal Betaine Triggers Bacterial Hydrogen Peroxide Production that Promotes Algal Demise.Nature communications · 2026Article
- Metacaspases contribute to the cellular response to heat stress in a marine diatom.The New phytologist · 2026Article
- Selenite reduces algal reactive oxygen species accumulation and enhances algal resistance to a bacterial pathogen.ISME communications · 2026Article
- Shrimp Virus Regulates ROS Dynamics via the Nrf2 Pathway to Facilitate Viral Replication.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Effects of oxidative stress on viral infections: an overview.Npj viruses · 2025Review
- Time of day of infection shapes development of a eukaryotic algal-Nucleocytoviricota virocell.FEMS microbiology ecology · 2024Article
- The consequences of viral infection on protists.Communications biology · 2024Review
- Cell-to-cell heterogeneity drives host-virus coexistence in a bloom-forming alga.The ISME journal · 2024Article
- UnveilingISME communications · 2024Article
- Daily turnover of active giant virus infection during algal blooms revealed by single-cell transcriptomics.Science advances · 2023Article
- Identification and Verification of Candidate miRNA Biomarkers with Application to Infection withGenes · 2023Article
- Visualization of RNA virus infection in a marine protist with a universal biomarker.Scientific reports · 2023Article
- A bacterial quorum sensing signal is a potent inhibitor ofFrontiers in microbiology · 2023Article
- Antiviral Potential of Melissa officinalis L.: A Literature Review.Nutrition and metabolic insights · 2023Review
- Flavobacterial exudates disrupt cell cycle progression and metabolism of the diatom Thalassiosira pseudonana.The ISME journal · 2022Article
- Viruses direct carbon cycling in lake sediments under global change.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- An Emiliania huxleyi pan-transcriptome reveals basal strain specificity in gene expression patterns.Scientific reports · 2021Article
- Viral infection of algal blooms leaves a unique metabolic footprint on the dissolved organic matter in the ocean.Science advances · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cosmopolitan coccolithophore Emiliania huxleyi is a unicellular eukaryotic alga responsible for vast blooms in the ocean. These blooms have immense impact on large biogeochemical cycles and are terminated by a specific large double-stranded DNA E. huxleyi virus (EhV, Phycodnaviridae). EhV infection is accompanied by induction of hallmarks of programmed cell death and production of reactive oxygen species (ROS). Here we characterized alterations in ROS metabolism and explored its role during infection. Transcriptomic analysis of ROS-related genes predicted an increase in glutathione (GSH) and H2O2 production during infection. In accordance, using biochemical assays and specific fluorescent probes we demonstrated the overproduction of GSH during lytic infection. We also showed that H2O2 production, rather than superoxide, is the predominant ROS during the onset of the lytic phase of infection. Using flow cytometry, confocal microscopy and multispectral imaging flow cytometry, we showed that the profound co-production of H2O2 and GSH occurred in the same subpopulation of cells but at different subcellular localization. Positively stained cells for GSH and H2O2 were highly infected compared with negatively stained cells. Inhibition of ROS production by application of a peroxidase inhibitor or an H2O2 scavenger inhibited host cell death and reduced viral production. We conclude that viral infection induced remodeling of the host antioxidant network that is essential for a successful viral replication cycle. This study provides insight into viral replication strategy and suggests the use of specific cellular markers to identify and quantify the extent of active viral infection during E. huxleyi blooms in the ocean.
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