ArticleMetabolites2016
Development of an Optimized Protocol for NMR Metabolomics Studies of Human Colon Cancer Cell Lines and First Insight from Testing of the Protocol Using DNA G-Quadruplex Ligands as Novel Anti-Cancer Drugs.
Article in Metabolites, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Dietary targeting of TRPM8 rewires macrophage immunometabolism reducing colitis severity.Cell death & disease · 2025Article
- uL3 Regulates Redox Metabolism and Ferroptosis Sensitivity of p53-Deleted Colorectal Cancer Cells.Antioxidants (Basel, Switzerland) · 2024Article
- Article
- Metabolomic Signatures in Doxorubicin-Induced Metabolites Characterization, Metabolic Inhibition, and Signaling Pathway Mechanisms in Colon Cancer HCT116 Cells.Metabolites · 2022Article
- Targeting Colorectal Cancer Cells with a Functionalised Calix[4]arene Receptor: Biophysical Studies.Molecules (Basel, Switzerland) · 2022Article
- Polystyrene microplastics induce gut microbiome and metabolome changes in Javanese medaka fish (Toxicology reports · 2022Article
- Xanthatin Induces Leishmanicidal Activity by Affecting Carbon Metabolism in Amastigotes.Iranian journal of pharmaceutical research : IJPR · 2021Article
- Article
- Metabolomic characterization of colorectal cancer cell lines highlighting stage-specific alterations during cancer progression.BioImpacts : BI · 2021Article
- Serum Metabolic Profiling Reveals the Antidepressive Effects of the Total Iridoids ofFrontiers in pharmacology · 2020Article
- Impact of phytosterols on liver and distal colon metabolome in experimental murine colitis model: an explorative study.Journal of enzyme inhibition and medicinal chemistry · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
The study of cell lines by nuclear magnetic resonance (NMR) spectroscopy metabolomics represents a powerful tool to understand how the local metabolism and biochemical pathways are influenced by external or internal stimuli. In particular, the use of adherent mammalian cells is emerging in the metabolomics field in order to understand the molecular mechanism of disease progression or, for example, the cellular response to drug treatments. Hereto metabolomics investigations for this kind of cells have generally been limited to mass spectrometry studies. This study proposes an optimized protocol for the analysis of the endo-metabolome of human colon cancer cells (HCT116) by NMR. The protocol includes experimental conditions such as washing, quenching and extraction. In order to test the proposed protocol, it was applied to an exploratory study of cancer cells with and without treatment by anti-cancer drugs, such as DNA G-quadruplex binders and Adriamycin (a traditional anti-cancer drug). The exploratory NMR metabolomics analysis resulted in NMR assignment of all endo-metabolites that could be detected and provided preliminary insights about the biological behavior of the drugs tested.
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