ArticleExperimental neurology2016
Generation of highly enriched V2a interneurons from mouse embryonic stem cells.
Article in Experimental neurology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Hyaluronic acid-alginate hydrazone crosslinked hydrogels support the generation and maturation of V2a interneurons.Journal of materials chemistry. B · 2026Article
- Effects of Complete Spinal Cord Transection in Neonatal and Juvenile Rats on Parvalbumin-Positive Inputs and Perineuronal Net Formation on Chx10-Positive V2a Interneurons.Developmental neuroscience · 2026Article
- Developmental stage of transplanted neural progenitor cells influences anatomical and functional outcomes after spinal cord injury in mice.Communications biology · 2023Article
- Chx10+V2a interneurons in spinal motor regulation and spinal cord injury.Neural regeneration research · 2023Review
- A transgenic mouse embryonic stem cell line for puromycin selection of V0Stem cell research & therapy · 2022Article
- Induction of Ventral Spinal V0 Interneurons from Mouse Embryonic Stem Cells.Stem cells and development · 2021Article
- Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications.Methods in molecular biology (Clifton, N.J.) · 2021Article
- V2a interneuron differentiation from mouse and human pluripotent stem cells.Nature protocols · 2019Article
- Transplanting Cells for Spinal Cord Repair: Who, What, When, Where and Why?Cell transplantation · 2019Article
- Choosing the right cell for spinal cord repair.Journal of neuroscience research · 2019Article
- Role of Propriospinal Neurons in Control of Respiratory Muscles and Recovery of Breathing Following Injury.Frontiers in systems neuroscience · 2019Review
- Derivation of Specific Neural Populations From Pluripotent Cells for Understanding and Treatment of Spinal Cord Injury.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- Transplantation of Neural Progenitors and V2a Interneurons after Spinal Cord Injury.Journal of neurotrauma · 2018Article
- The Neuroplastic and Therapeutic Potential of Spinal Interneurons in the Injured Spinal Cord.Trends in neurosciences · 2018Review
- Genome engineering for CNS injury and disease.Current opinion in biotechnology · 2018Review
- Effect of hyaluronic acid hydrogels containing astrocyte-derived extracellular matrix and/or V2a interneurons on histologic outcomes following spinal cord injury.Biomaterials · 2018Article
- Adult Neural Stem Cells: Basic Research and Production Strategies for Neurorestorative Therapy.Stem cells international · 2018Review
- Regulatory networks specifying cortical interneurons from human embryonic stem cells reveal roles for CHD2 in interneuron development.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Different Mixed Astrocyte Populations Derived from Embryonic Stem Cells Have Variable Neuronal Growth Support Capacities.Stem cells and development · 2017Article
- Differentiation of V2a interneurons from human pluripotent stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Challenges in parsing specific contributions to spinal microcircuit architecture have limited our ability to model and manipulate those networks for improved functional regeneration after injury or disease. While spinal interneurons (INs) have been implicated in driving coordinated locomotor behaviors, they constitute only a small percentage of the spinal cord and are difficult to isolate from primary tissue. In this study, we employed a genetic strategy to obtain large quantities of highly enriched mouse embryonic stem cell (ESC)-derived V2a INs, an excitatory glutamatergic IN population that is defined by expression of the homeodomain protein Chx10 during development. Puromycin N-acetyltransferase expression was driven by the native gene regulatory elements of Chx10 in the transgenic ESC line, resulting in positive selection of V2a INs after induction and treatment with puromycin. Directly after selection, approximately 80% of cells are Chx10(+), with 94% Lhx3(+); after several weeks, cultures remain free of proliferative cell types and mature into normal glutamatergic neurons as assessed by molecular markers and electrophysiological methods. Functional synapses were observed between selected ESC-derived V2a INs and motor neurons when co-cultured, demonstrating the potential of these cells to form neural networks. While ESC-derived neurons obtained in vitro are not identical to those that develop in the spinal cord, the transgenic ESCs here provide a unique tool to begin studying V2a INs in isolation or for use in in vitro models of spinal microcircuits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.