ArticleThe Journal of biological chemistry2016
Crystal Structure of Manganese Lipoxygenase of the Rice Blast Fungus Magnaporthe oryzae.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 69 citations in OpenAlex.
- 8-Iso-Prostaglandin F2α and Lipoxygenase Gene Expression as Candidate Molecular Markers of Training Adaptation in Professional Volleyball Players: A Pilot Study.International journal of molecular sciences · 2026Article
- Activation and execution of lipoxygenase catalysis.Redox biochemistry and chemistry · 2026Article
- Allosteric properties of mammalian ALOX15 orthologs.The Journal of biological chemistry · 2026Review
- Biochemical Consequences of a Leucine-to-Cysteine Clamp Substitution in Lipoxygenases.Biomolecules · 2025Article
- Review
- Synthesis, characterization and reactivity of a Mn(III)-hydroxido complex as a biomimetic model for lipoxygenase.Journal of inorganic biochemistry · 2024Article
- Impact ofBiochemistry · 2024Article
- Antifungal plant flavonoids identified in silico with potential to control rice blast disease caused by Magnaporthe oryzae.PloS one · 2024Article
- Article
- Genome-wide identification and analysis ofFrontiers in genetics · 2022Article
- Mimicking Elementary Reactions of Manganese Lipoxygenase Using Mn-hydroxo and Mn-alkylperoxo Complexes.Molecules (Basel, Switzerland) · 2021Article
- Biochemical Characterization of 13-Lipoxygenases ofInternational journal of molecular sciences · 2021Article
- Genomic Analyses ofJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Concerted proton-electron transfer reactions of manganese-hydroxo and manganese-oxo complexes.Chemical communications (Cambridge, England) · 2020Article
- Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases.Molecules (Basel, Switzerland) · 2020Review
- The Soybean Lipoxygenase-Substrate Complex: Correlation between the Properties of Tunneling-Ready States and ENDOR-Detected Structures of Ground States.Biochemistry · 2020Article
- An ensemble of lipoxygenase structures reveals novel conformations of the Fe coordination sphere.Protein science : a publication of the Protein Society · 2019Article
- Structural, Spectroscopic, Electrochemical, and Magnetic Properties for Manganese(II) Triazamacrocyclic Complexes.Inorganica chimica acta · 2019Article
- Stabilization and improved activity of arachidonate 11Journal of lipid research · 2018Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipoxygenases (LOX) are non-heme metal enzymes, which oxidize polyunsaturated fatty acids to hydroperoxides. All LOX belong to the same gene family, and they are widely distributed. LOX of animals, plants, and prokaryotes contain iron as the catalytic metal, whereas fungi express LOX with iron or with manganese. Little is known about metal selection by LOX and the adjustment of the redox potentials of their protein-bound catalytic metals. Thirteen three-dimensional structures of animal, plant, and prokaryotic FeLOX are available, but none of MnLOX. The MnLOX of the most important plant pathogen, the rice blast fungusMagnaporthe oryzae(Mo), was expressed inPichia pastoris.Mo-MnLOX was deglycosylated, purified to homogeneity, and subjected to crystal screening and x-ray diffraction. The structure was solved by sulfur and manganese single wavelength anomalous dispersion to a resolution of 2.0 Å. The manganese coordinating sphere is similar to iron ligands of coral 8R-LOX and soybean LOX-1 but is not overlapping. The Asn-473 is positioned on a short loop (Asn-Gln-Gly-Glu-Pro) instead of an α-helix and forms hydrogen bonds with Gln-281. Comparison with FeLOX suggests that Phe-332 and Phe-525 might contribute to the unique suprafacial hydrogen abstraction and oxygenation mechanism of Mo-MnLOX by controlling oxygen access to the pentadiene radical. Modeling suggests that Arg-525 is positioned close to Arg-182 of 8R-LOX, and both residues likely tether the carboxylate group of the substrate. An oxygen channel could not be identified. We conclude that Mo-MnLOX illustrates a partly unique variation of the structural theme of FeLOX.
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