ArticleNature communications2016
Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Accurate prediction of oligonucleotide probe hybridization efficiency via a transformer-based model.BMC genomics · 2026Article
- Kinetics and Activation Strategies in Toehold-Mediated and Toehold-Free DNA Strand Displacement.Biosensors · 2025Review
- Predicting DNA Reactions with a Quantum Chemistry-Based Deep Learning Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution.Sensors (Basel, Switzerland) · 2023Article
- Best practice for improved accuracy: A critical reassessment of van't Hoff analysis of melt curves.Biophysical journal · 2022Article
- High-throughput methods for measuring DNA thermodynamics.Nucleic acids research · 2020Article
- Encoding multiple digital DNA signals in a single analog channel.Nucleic acids research · 2020Article
- Elucidation of leak-resistance DNA hybridization chain reaction with universality and extensibility.Nucleic acids research · 2020Article
- Target-Triggered Polymerization of Branched DNA Enables Enzyme-free and Fast Discrimination of Single-Base Changes.iScience · 2019Article
- Simulation-guided engineering of an enzyme-powered three dimensional DNA nanomachine for discriminating single nucleotide variants.Chemical science · 2018Article
- Dihydropyridine Fluorophores Allow for Specific Detection of Human Antibodies in Serum.ACS omega · 2018Article
- Determining the folding and binding free energy of DNA-based nanodevices and nanoswitches using urea titration curves.Nucleic acids research · 2017Article
- Multiplexed enrichment of rare DNA variants via sequence-selective and temperature-robust amplification.Nature biomedical engineering · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA hybridization thermodynamics is critical for accurate design of oligonucleotides for biotechnology and nanotechnology applications, but parameters currently in use are inaccurately extrapolated based on limited quantitative understanding of thermal behaviours. Here, we present a method to measure the ΔG° of DNA motifs at temperatures and buffer conditions of interest, with significantly better accuracy (6- to 14-fold lower s.e.) than prior methods. The equilibrium constant of a reaction with thermodynamics closely approximating that of a desired motif is numerically calculated from directly observed reactant and product equilibrium concentrations; a DNA catalyst is designed to accelerate equilibration. We measured the ΔG° of terminal fluorophores, single-nucleotide dangles and multinucleotide dangles, in temperatures ranging from 10 to 45 °C.
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