ArticleBiomedical microdevices2016
Protein patterning by microcontact printing using pyramidal PDMS stamps.
Article in Biomedical microdevices, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Strategies for Multiplexing Plasmonic Biosensing.Sensors (Basel, Switzerland) · 2026Review
- A programmable peptide interface for on-demand neural culturing platforms.Journal of nanobiotechnology · 2026Article
- Physics-informed displacement control for variable pattern printing with V-shaped PDMS stamps in roll-to-roll microcontact printing.Communications engineering · 2025Article
- Soft lithography-based biomolecule patterning techniques and their applications in subcellular protein interaction analysis.Materials today. Bio · 2025Review
- Protein Orientation and Polymer Phase Separation Induced by Poly(methyl methacrylate) Tacticity.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Hydrophobic Recovery of PDMS Surfaces in Contact with Hydrophilic Entities: Relevance to Biomedical Devices.Materials (Basel, Switzerland) · 2022Article
- Advances in Single-Cell Printing.Micromachines · 2022Review
- Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance.Frontiers in cell and developmental biology · 2021Article
- Lab-on-a-Chip for Cardiovascular Physiology and Pathology.Micromachines · 2020Review
- Exploiting CRISPR Cas9 in Three-Dimensional Stem Cell Cultures to Model Disease.Frontiers in bioengineering and biotechnology · 2020Review
- Fabrication, Characterization and Application of Biomolecule Micropatterns on Cyclic Olefin Polymer (COP) Surfaces with Adjustable Contrast.Biosensors · 2019Article
- Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications.Bioengineering (Basel, Switzerland) · 2019Review
- A FRET assay for the quantitation of inhibitors of exonuclease EcoRV by using parchment paper inkjet-printed with graphene oxide and FAM-labelled DNA.Mikrochimica acta · 2019Article
- Design Principles for Pluripotent Stem Cell-Derived Organoid Engineering.Stem cells international · 2019Review
- Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays.PloS one · 2018Article
- A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns.Frontiers in chemistry · 2018Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Micro-contact printing, μCP, is a well-established soft-lithography technique for printing biomolecules. μCP uses stamps made of Poly(dimethylsiloxane), PDMS, made by replicating a microstructured silicon master fabricated by semiconductor manufacturing processes. One of the problems of the μCP is the difficult control of the printing process, which, because of the high compressibility of PDMS, is very sensitive to minute changes in the applied pressure. This over-sensitive response leads to frequent and/or uncontrollable collapse of the stamps with high aspect ratios, thus decreasing the printing accuracy and reproducibility. Here we present a straightforward methodology of designing and fabricating PDMS structures with an architecture which uses the collapse of the stamp to reduce, rather than enlarge the variability of the printing. The PDMS stamp, organized as an array of pyramidal micro-posts, whose ceiling collapses when pressed on a flat surface, replicates the structure of the silicon master fabricated by anisotropic wet etching. Upon application of pressure, depending on the size of, and the pitch between, the PDMS pyramids, an air gap is formed surrounding either the entire array, or individual posts. The printing technology, which also exhibits a remarkably low background noise for fluorescence detection, may find applications when the clear demarcation of the shapes of protein patterns and the distance between them are critical, such as microarrays and studies of cell patterning.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.