ArticleScientific reports2016
Rspo1-activated signalling molecules are sufficient to induce ovarian differentiation in XY medaka (Oryzias latipes).
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Sex-specific differences in zebrafish brains.Biology of sex differences · 2022Pooled it
- Integrative Methylome and Transcriptome Analysis of DNA Methylation and Sex-Biased Gene Expression in Northern Pike (Animals : an open access journal from MDPI · 2026Article
- Stage-Specific Transcriptomic Analysis Reveals Molecular Basis of Ovarian Sterility in Triploid Turbot (Animals : an open access journal from MDPI · 2026Article
- The Heterogeneous Nuclear Ribonucleoprotein K (Animals : an open access journal from MDPI · 2026Article
- Before the East African Radiation: Sex Chromosome Systems in Basal Haplotilapiine Cichlids.Genome biology and evolution · 2026Article
- Whole-Genome Methylation Analysis of Female, Male, and Neomale Northern Pike (Animals : an open access journal from MDPI · 2025Article
- Female-Biased Expression ofAnimals : an open access journal from MDPI · 2023Article
- Isolation and Characterization of Germline Stem Cells in Protogynous HermaphroditicInternational journal of molecular sciences · 2022Article
- Becoming female: Ovarian differentiation from an evolutionary perspective.Frontiers in cell and developmental biology · 2022Review
- The replaceable master of sex determination: bottom-up hypothesis revisited.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.Cell death and differentiation · 2020Article
- Whole genome-wide chromosome fusion and new gene birth in theCell & bioscience · 2020Article
- Expression ofAnimals : an open access journal from MDPI · 2019Article
- Estrogen Receptor β2 Oversees Germ Cell Maintenance and Gonadal Sex Differentiation in Medaka, Oryzias latipes.Stem cell reports · 2019Article
- A Comparative View on Sex Differentiation and Gametogenesis Genes in Lungfish and Coelacanths.Genome biology and evolution · 2018Article
- Integrated analysis of mRNA-seq and miRNA-seq reveals the potential roles of sex-biased miRNA-mRNA pairs in gonad tissue of dark sleeper (Odontobutis potamophila).BMC genomics · 2017Article
- Histological and transcriptomic effects of 17α-methyltestosterone on zebrafish gonad development.BMC genomics · 2017Article
- Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision.Scientific reports · 2017Article
- Germline stem cells are critical for sexual fate decision of germ cells.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Dmy initiates masculinity by altering Gsdf/Sox9a2/Rspo1 expression in medaka (Oryzias latipes).Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to our understanding of testicular differentiation, ovarian differentiation is less well understood in vertebrates. In mammals, R-spondin1 (Rspo1), an activator of Wnt/β-catenin signaling pathway, is located upstream of the female sex determination pathway. However, the functions of Rspo1 in ovarian differentiation remain unclear in non-mammalian species. In order to elucidate the detailed functions of Rspo/Wnt signaling pathway in fish sex determination/differentiation, the ectopic expression of the Rspo1 gene was performed in XY medaka (Oryzias latipes). The results obtained demonstrated that the gain of Rspo1 function induced femininity in XY fish. The overexpression of Rspo1 enhanced Wnt4b and β-catenin transcription, and completely suppressed the expression of male-biased genes (Dmy, Gsdf, Sox9a2 and Dmrt1) as well as testicular differentiation. Gonadal reprograming of Rspo1-over-expressed-XY (Rspo1-OV-XY) fish, induced the production of female-biased genes (Cyp19a1a and Foxl2), estradiol-17β production and further female type secondary sexuality. Moreover, Rspo1-OV-XY females were fertile and produced successive generations. Promoter analyses showed that Rspo1 transcription was directly regulated by DM domain genes (Dmy, the sex-determining gene, and Dmrt1) and remained unresponsive to Foxl2. Taken together, our results strongly suggest that Rspo1 is sufficient to activate ovarian development and plays a decisive role in the ovarian differentiation in medaka.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.