Evidence map›Paper›PMID 26782178›Full record

ArticleStem cells (Dayton, Ohio)2016

Comprehensive Proteomic Analysis of Mesenchymal Stem Cell Exosomes Reveals Modulation of Angiogenesis via Nuclear Factor-KappaB Signaling.

Johnathon D Anderson, Henrik J Johansson, Calvin S Graham, Mattias Vesterlund, Missy T Pham, Charles S Bramlett, Elizabeth N Montgomery, Matt S Mellema, Renee L Bardini, Zelenia Contreras and 11 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 303 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
303citing papers in PubMed, 4 pooled it
29.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

303 citing papers in PubMed, 4 syntheses or guidelines pooled it, 531 citations in OpenAlex.

  1. Exploring the landscape of stem cell extracellular vesicle research for angiogenesis: A bibliometric analysis from 2003 to 2023.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024
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  19. 80 years of extracellular vesicles: from discovery to clinical translation.Extracellular vesicles and circulating nucleic acids · 2026
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243 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 3 institutions in 3 countries.

Johnathon D AndersonStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Henrik J JohanssonCancer Proteomics, Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
Calvin S GrahamStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Mattias VesterlundCancer Proteomics, Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
Missy T PhamStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Charles S BramlettStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Elizabeth N MontgomeryStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Matt S MellemaSurgical and Radiological Sciences, Department of Veterinary Medicine, University of California Davis, Davis, California, USA.
Renee L BardiniStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Zelenia ContrerasStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Madeline HoonStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Gerhard BauerStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Kyle D FinkStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Brian FuryStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Kyle J HendrixStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
Frederic ChedinDepartment of Molecular and Cellular Biology, University of California Davis, Davis, California, USA.
Samir El-AndaloussiDepartment of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.
Billie HwangDepartment of Surgery, University of Washington, Seattle, Washington, USA.
Michael S MulliganDepartment of Surgery, University of Washington, Seattle, Washington, USA.
Janne LehtiöCancer Proteomics, Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden.
Jan A NoltaStem Cell Program, Department of Internal Medicine, University of California Davis, Davis, California, USA.
University of California, Davis · USKarolinska Institutet · SEUniversity of Washington · US

Funding

Training Program In Basic & Translational Cardiovascular ScienceT32HL086350 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Martin Cadeiras, David A. Liem · 2008 to 2026
$7.1M
Direct cell to cell transfer of microRNA for tissue repairR01GM099688 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI NOLTA, JAN A. · 2011 to 2015
$2.7M
Training Program in Biomolecular TechnologyT32GM008799 · NIGMS · UNIVERSITY OF CALIFORNIA DAVIS · PI LEACH, J. KENT · 2002 to 2016
$2.4M
Predoctoral Training in Pharmacological SciencesT32GM099608 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI HELL, JOHANNES W · 2012 to 2021
$2.2M
NHLBI NIH HHS T32 HL086350NIGMS NIH HHS R01 GM099688NIGMS NIH HHS R01GM099688NIGMS NIH HHS T32 GM008799NIGMS NIH HHS T32-GM008799NIGMS NIH HHS T32 GM099608
6 · The paper itself

Abstract

Mesenchymal stem cells (MSC) are known to facilitate healing of ischemic tissue related diseases through proangiogenic secretory proteins. Recent studies further show that MSC derived exosomes function as paracrine effectors of angiogenesis, however, the identity of which components of the exosome proteome responsible for this effect remains elusive. To address this we used high-resolution isoelectric focusing coupled liquid chromatography tandem mass spectrometry, an unbiased high throughput proteomics approach to comprehensively characterize the proteinaceous contents of MSCs and MSC derived exosomes. We probed the proteome of MSCs and MSC derived exosomes from cells cultured under expansion conditions and under ischemic tissue simulated conditions to elucidate key angiogenic paracrine effectors present and potentially differentially expressed in these conditions. In total, 6,342 proteins were identified in MSCs and 1,927 proteins in MSC derived exosomes, representing to our knowledge the first time these proteomes have been probed comprehensively. Multilayered analyses identified several putative paracrine effectors of angiogenesis present in MSC exosomes and increased in expression in MSCs exposed to ischemic tissue-simulated conditions; these include platelet derived growth factor, epidermal growth factor, fibroblast growth factor, and most notably nuclear factor-kappaB (NFkB) signaling pathway proteins. NFkB signaling was identified as a key mediator of MSC exosome induced angiogenesis in endothelial cells by functional in vitro validation using a specific inhibitor. Collectively, the results of our proteomic analysis show that MSC derived exosomes contain a robust profile of angiogenic paracrine effectors, which have potential for the treatment of ischemic tissue-related diseases.

Indexed as

Bone Marrow CellsExosomesGene Expression Regulation, DevelopmentalHumansMaleMesenchymal Stem CellsNeovascularization, PhysiologicNF-kappa BParacrine CommunicationProteomeSignal TransductionYoung AdultNF-kappa BProteomeExosomesHigh-resolution isoelectric focusingLiquid chromatography tandem mass spectrometryMesenchymal stem cellsNuclear factor kappaBPeripheral arterial diseaseProteomics

Identifiers

PMID26782178
PMCPMC5785927
OpenAlexW2264590390

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.