ArticleScientific reports2016
Molecular Combing of Single DNA Molecules on the 10 Megabase Scale.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Microfluidic delivery of cutting enzymes for fragmentation of surface-adsorbed DNA molecules.PloS one · 2023Article
- DNA replication profiling by molecular combing on single DNA fibers.STAR protocols · 2022Article
- A simple cut and stretch assay to detect antimicrobial resistance genes on bacterial plasmids by single-molecule fluorescence microscopy.Scientific reports · 2022Article
- Genome-wide mapping of human DNA replication by optical replication mapping supports a stochastic model of eukaryotic replication.Molecular cell · 2021Article
- DNA bridges: A novel platform for single-molecule sequencing and other DNA-protein interaction applications.PloS one · 2021Article
- Single-Molecule Techniques to Study Chromatin.Frontiers in cell and developmental biology · 2021Review
- Time for remodeling: SNF2-family DNA translocases in replication fork metabolism and human disease.DNA repair · 2020Review
- On-Chip Stretching, Sorting, and Electro-Optical Nanopore Sensing of Ultralong Human Genomic DNA.ACS nano · 2019Article
- A micropatterned substrate for on-surface enzymatic labelling of linearized long DNA molecules.Scientific reports · 2019Article
- DNA barcodes for rapid, whole genome, single-molecule analyses.Nucleic acids research · 2019Article
- Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA.Molecules (Basel, Switzerland) · 2019Review
- DNA Replication: From Radioisotopes to Click Chemistry.Molecules (Basel, Switzerland) · 2018Review
- Human Papillomavirus Integration: Analysis by Molecular Combing and Fiber-FISH.Current protocols in microbiology · 2018Article
- Spatial separation between replisome- and template-induced replication stress signaling.The EMBO journal · 2018Article
- Genome-Wide Analysis of the Arabidopsis Replication Timing Program.Plant physiology · 2018Article
- CDK activity provides temporal and quantitative cues for organizing genome duplication.PLoS genetics · 2018Article
- DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.Nature communications · 2017Article
- MTBP, the partner of Treslin, contains a novel DNA-binding domain that is essential for proper initiation of DNA replication.Molecular biology of the cell · 2017Article
- Single-molecule Analysis of DNA Replication Dynamics in Budding Yeast and Human Cells by DNA Combing.Bio-protocol · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
DNA combing allows the investigation of DNA replication on genomic single DNA molecules, but the lengths that can be analysed have been restricted to molecules of 200-500 kb. We have improved the DNA combing procedure so that DNA molecules can be analysed up to the length of entire chromosomes in fission yeast and up to 12 Mb fragments in human cells. Combing multi-Mb-scale DNA molecules revealed previously undetected origin clusters in fission yeast and shows that in human cells replication origins fire stochastically forming clusters of fired origins with an average size of 370 kb. We estimate that a single human cell forms around 3200 clusters at mid S-phase and fires approximately 100,000 origins to complete genome duplication. The procedure presented here will be adaptable to other organisms and experimental conditions.
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