ArticleBMC musculoskeletal disorders2016
Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping.
Article in BMC musculoskeletal disorders, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
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- Transcriptional Profiling of Muscle in Females With Distal Radius Fracture and Functional Sarcopenia.The journals of gerontology. Series A, Biological sciences and medical sciences · 2024Article
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- Osteopathy in Complex Lymphatic Anomalies.International journal of molecular sciences · 2022Review
- Tendon healing is adversely affected by low-grade inflammation.Journal of orthopaedic surgery and research · 2021Review
- Inflammatory mechanisms linking obesity and tendinopathy.Journal of orthopaedic translation · 2021Review
- Tendinopathy and tendon material response to load: What we can learn from small animal studies.Acta biomaterialia · 2021Review
- Force dependent effects of chronic overuse on fibrosis-related genes and proteins in skeletal muscles.Connective tissue research · 2021Article
- Blocking substance P signaling reduces musculotendinous and dermal fibrosis and sensorimotor declines in a rat model of overuse injury.Connective tissue research · 2020Article
- Article
- Cytokines in tendon disease: A Systematic Review.Bone & joint research · 2017Article
- Decreased grip strength, muscle pain, and atrophy occur in rats following long-term exposure to excessive repetitive motion.FEBS open bio · 2017Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundExpression of the growth factor osteoactivin (OA) increases during tissue degeneration and regeneration, fracture repair and after denervation-induced disuse atrophy, concomitant with increased matrix metalloproteinases (MMPs). However, OA's expression with repetitive overuse injuries is unknown. The aim of this study was to evaluate: 1) OA expression in an operant rat model of repetitive overuse; 2) expression of MMPs; 3) inflammatory cytokines indicative of injury or inflammation; and 4) the inducible form of heat shock protein 70 (HSPA1A/HSP72) as the latter is known to increase during metabolic stress and to be involved in cellular repair. Young adult female rats performed a high repetition negligible force (HRNF) food retrieval task for up to 6 weeks and were compared to control rats.
methodsFlexor digitorum muscles and tendons were collected from 22 young adult female rats performing a HRNF reaching task for 3 to 6 weeks, and 12 food restricted control (FRC) rats. OA mRNA levels were assessed by quantitative polymerase chain reaction (qPCR). OA, MMP-1, -2, -3, and -13 and HSP72 protein expression was assayed using Western blotting. Immunohistochemistry and image analysis was used to evaluate OA and HSP72 expression. ELISA was performed for HSP72 and inflammatory cytokines.
resultsFlexor digitorum muscles and tendons from 6-week HRNF rats showed increased OA mRNA and protein expression compared to FRC rats. MMP-1, -2 and -3 progressively increased in muscles whereas MMP-1 and -3 increased in tendons with HRNF task performance. HSP72 increased in 6-week HRNF muscles and tendons, compared to controls, and co-localized with OA in the myofiber sarcolemma. IL-1alpha and beta increased transiently in tendons or muscles in HRNF week 3 before resolving in week 6.
conclusionThe simultaneous increases of OA with factors involved in tissue repair (MMPs and HSP72) supports a role of OA in tissue regeneration after repetitive overuse.
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